PIX-GIT复合体：一种控制细胞形状的G蛋白信号转导组件
The PIX-GIT complex: a G protein signaling cassette in control of cell shape.
作者信息
Frank Scott R, Hansen Steen H
机构信息
GI Cell Biology Laboratory, The Children's Hospital, Harvard Medical School, Boston, MA 02115, USA.
出版信息
Semin Cell Dev Biol. 2008 Jun;19(3):234-44. doi: 10.1016/j.semcdb.2008.01.002. Epub 2008 Jan 20.
Abstract
Arf and Rho GTP-binding proteins coordinately regulate membrane dynamics and cytoskeletal rearrangements. The Cdc42/Rac guanine nucleotide exchange factor PIX and the Arf GTPase-activating protein GIT form a stable complex in cells. The PIX-GIT complex functions to integrate signaling among Arf, Cdc42, and Rac proteins in response to cues emanating from integrins, heterotrimeric G proteins, receptor tyrosine kinases, and cell-cell interactions. A concept that emerges from the literature is that the PIX-GIT complex serves as a cassette to elicit changes in cell shape essential for polarized cell responses in a wide range of biological contexts.
摘要
Arf和Rho GTP结合蛋白协同调节膜动力学和细胞骨架重排。Cdc42/Rac鸟嘌呤核苷酸交换因子PIX与Arf GTP酶激活蛋白GIT在细胞中形成稳定复合物。PIX - GIT复合物的功能是整合来自整合素、异源三聚体G蛋白、受体酪氨酸激酶和细胞间相互作用发出的信号，以在Arf、Cdc42和Rac蛋白之间传递信号。文献中出现的一个概念是，PIX - GIT复合物作为一个组件，在广泛的生物学背景下引发细胞形状变化，这对极化细胞反应至关重要。
相似文献
1
The PIX-GIT complex: a G protein signaling cassette in control of cell shape.PIX-GIT复合体：一种控制细胞形状的G蛋白信号转导组件
Semin Cell Dev Biol. 2008 Jun;19(3):234-44. doi: 10.1016/j.semcdb.2008.01.002. Epub 2008 Jan 20.
2
[The GIT-PIX protein complex: a hub to ARF and Rac/Cdc42 GTPases].[胃肠道-PIX蛋白复合物：ARF及Rac/Cdc42 GTP酶的枢纽]
Med Sci (Paris). 2005 Oct;21(10):849-53. doi: 10.1051/medsci/20052110849.
3
Expanding functions of GIT Arf GTPase-activating proteins, PIX Rho guanine nucleotide exchange factors and GIT-PIX complexes.胃肠道Arf GTP酶激活蛋白、PIX Rho鸟嘌呤核苷酸交换因子和GIT-PIX复合物的功能扩展
J Cell Sci. 2016 May 15;129(10):1963-74. doi: 10.1242/jcs.179465.
4
The GIT/PIX complex: an oligomeric assembly of GIT family ARF GTPase-activating proteins and PIX family Rac1/Cdc42 guanine nucleotide exchange factors.GIT/PIX复合物：一种由GIT家族ARF GTP酶激活蛋白和PIX家族Rac1/Cdc42鸟嘌呤核苷酸交换因子组成的寡聚体。
Cell Signal. 2004 Sep;16(9):1001-11. doi: 10.1016/j.cellsig.2004.02.002.
5
Role of phospholipase Cgamma1 in cell spreading requires association with a beta-Pix/GIT1-containing complex, leading to activation of Cdc42 and Rac1.磷脂酶Cγ1在细胞铺展中的作用需要与含β-Pix/GIT1的复合物结合，从而导致Cdc42和Rac1的激活。
Mol Cell Biol. 2007 Aug;27(16):5790-805. doi: 10.1128/MCB.00778-07. Epub 2007 Jun 11.
6
Structures of dimeric GIT1 and trimeric beta-PIX and implications for GIT-PIX complex assembly.二聚体GIT1和三聚体β-PIX的结构及其对GIT-PIX复合物组装的影响。
J Mol Biol. 2009 Feb 20;386(2):280-9. doi: 10.1016/j.jmb.2008.12.050. Epub 2008 Dec 30.
7
The X-linked lymphoproliferative disease gene product SAP associates with PAK-interacting exchange factor and participates in T cell activation.X连锁淋巴增殖性疾病基因产物SAP与PAK相互作用交换因子结合并参与T细胞活化。
Proc Natl Acad Sci U S A. 2006 Sep 26;103(39):14447-52. doi: 10.1073/pnas.0606624103. Epub 2006 Sep 18.
8
Structural analysis of the SH3 domain of beta-PIX and its interaction with alpha-p21 activated kinase (PAK).β-PIX的SH3结构域的结构分析及其与α-p21激活激酶（PAK）的相互作用。
Biochemistry. 2005 Aug 23;44(33):10977-83. doi: 10.1021/bi050374a.
9
Protein kinase A-dependent phosphorylation modulates beta1Pix guanine nucleotide exchange factor activity through 14-3-3beta binding.蛋白激酶A依赖性磷酸化通过与14-3-3β结合来调节β1Pix鸟嘌呤核苷酸交换因子的活性。
Mol Cell Biol. 2008 Mar;28(5):1679-87. doi: 10.1128/MCB.00898-07. Epub 2007 Dec 26.
10
Coupling of PAK-interacting exchange factor PIX to GIT1 promotes focal complex disassembly.PAK相互作用交换因子PIX与GIT1的偶联促进粘着斑复合体的解体。
Mol Cell Biol. 2000 Sep;20(17):6354-63. doi: 10.1128/MCB.20.17.6354-6363.2000.
引用本文的文献
1
Regulation of microtubule nucleation in glioblastoma cells by ARF GTPase-activating proteins GIT1 and GIT2 and protein kinase C.ARF GTP酶激活蛋白GIT1和GIT2以及蛋白激酶C对胶质母细胞瘤细胞中微管成核的调控
Cancer Cell Int. 2025 Apr 2;25(1):125. doi: 10.1186/s12935-025-03740-y.
2
Modulating PAK1: Accessory Proteins as Promising Therapeutic Targets.调控PAK1：辅助蛋白作为有前景的治疗靶点
Biomolecules. 2025 Feb 7;15(2):242. doi: 10.3390/biom15020242.
3
The Discovery of GIT1/β-Pix Inhibitors: Virtual Screening and Biological Evaluation of New Small-molecule Compounds with Anti-invasion Effect in Gastrointestinal Neoplasms.GIT1/β-Pix 抑制剂的发现：具有抗胃肠道肿瘤侵袭作用的新型小分子化合物的虚拟筛选和生物学评价。
Drug Des Devel Ther. 2024 Jul 16;18:3075-3088. doi: 10.2147/DDDT.S461609. eCollection 2024.
4
Regulation of microtubule nucleation in mouse bone marrow-derived mast cells by ARF GTPase-activating protein GIT2.ARF GTPase-activating protein GIT2 调控小鼠骨髓来源的肥大细胞中的微管起始
Front Immunol. 2024 Feb 2;15:1321321. doi: 10.3389/fimmu.2024.1321321. eCollection 2024.
5
The Drosophila tumour suppressor Lgl and Vap33 activate the Hippo pathway through a dual mechanism.果蝇肿瘤抑制因子 Lgl 和 Vap33 通过双重机制激活 Hippo 通路。
J Cell Sci. 2024 Feb 15;137(4). doi: 10.1242/jcs.261917. Epub 2024 Feb 16.
6
Macrophage Function Modulated by tPA Signaling in Mouse Experimental Kidney Disease Models.tPA 信号对小鼠实验性肾病模型中巨噬细胞功能的调节。
Int J Mol Sci. 2023 Jul 4;24(13):11067. doi: 10.3390/ijms241311067.
7
Membrane adhesion junctions regulate airway smooth muscle phenotype and function.膜黏附连接调控气道平滑肌表型和功能。
Physiol Rev. 2023 Jul 1;103(3):2321-2347. doi: 10.1152/physrev.00020.2022. Epub 2023 Feb 16.
8
β-PIX cooperates with GIT1 to regulate endothelial nitric oxide synthase in sinusoidal endothelial cells.β-PIX 与 GIT1 合作调节窦状内皮细胞中的内皮型一氧化氮合酶。
Am J Physiol Gastrointest Liver Physiol. 2022 Nov 1;323(5):G511-G522. doi: 10.1152/ajpgi.00034.2022. Epub 2022 Aug 31.
9
Beta-Pix-dynamin 2 complex promotes colorectal cancer progression by facilitating membrane dynamics.β-Pix-动力蛋白 2 复合物通过促进膜动力学促进结直肠癌细胞的进展。
Cell Oncol (Dordr). 2021 Dec;44(6):1287-1305. doi: 10.1007/s13402-021-00637-6. Epub 2021 Sep 28.
10
A kinase-independent function of PAK is crucial for pathogen-mediated actin remodelling.PAK 的激酶非依赖性功能对于病原体介导的肌动蛋白重塑至关重要。
PLoS Pathog. 2021 Aug 30;17(8):e1009902. doi: 10.1371/journal.ppat.1009902. eCollection 2021 Aug.
本文引用的文献
1
Identification of an intramolecular interaction important for the regulation of GIT1 functions.鉴定对GIT1功能调节至关重要的分子内相互作用。
Mol Biol Cell. 2007 Dec;18(12):5124-38. doi: 10.1091/mbc.e07-06-0550. Epub 2007 Sep 26.
2
An ACAP1-containing clathrin coat complex for endocytic recycling.一种用于内吞再循环的含ACAP1的网格蛋白包被复合体。
J Cell Biol. 2007 Jul 30;178(3):453-64. doi: 10.1083/jcb.200608033.
3
Arf6 recruits the Rac GEF Kalirin to the plasma membrane facilitating Rac activation.Arf6将Rac鸟嘌呤核苷酸交换因子Kalirin招募至质膜，促进Rac激活。
BMC Cell Biol. 2007 Jul 18;8:29. doi: 10.1186/1471-2121-8-29.
4
A betaPix Pak2a signaling pathway regulates cerebral vascular stability in zebrafish.一条βPix-Pak2a信号通路调节斑马鱼脑血管的稳定性。
Proc Natl Acad Sci U S A. 2007 Aug 28;104(35):13990-5. doi: 10.1073/pnas.0700825104. Epub 2007 Jun 15.
5
Differential expression of the ARF GAP genes GIT1 and GIT2 in mouse tissues.ARF GAP基因GIT1和GIT2在小鼠组织中的差异表达。
J Histochem Cytochem. 2007 Oct;55(10):1039-48. doi: 10.1369/jhc.7A7207.2007. Epub 2007 Jun 12.
6
Role of phospholipase Cgamma1 in cell spreading requires association with a beta-Pix/GIT1-containing complex, leading to activation of Cdc42 and Rac1.磷脂酶Cγ1在细胞铺展中的作用需要与含β-Pix/GIT1的复合物结合，从而导致Cdc42和Rac1的激活。
Mol Cell Biol. 2007 Aug;27(16):5790-805. doi: 10.1128/MCB.00778-07. Epub 2007 Jun 11.
7
GIT1 utilizes a focal adhesion targeting-homology domain to bind paxillin.GIT1利用一个粘着斑靶向同源结构域来结合桩蛋白。
Cell Signal. 2007 Aug;19(8):1733-44. doi: 10.1016/j.cellsig.2007.03.010. Epub 2007 Mar 30.
8
Induction of vascular permeability: beta PIX and GIT1 scaffold the activation of extracellular signal-regulated kinase by PAK.血管通透性的诱导：βPIX和GIT1介导PAK对细胞外信号调节激酶的激活。
Mol Biol Cell. 2007 Jun;18(6):2346-55. doi: 10.1091/mbc.e06-07-0584. Epub 2007 Apr 11.
9
Interaction between the Helicobacter pylori CagA and alpha-Pix in gastric epithelial AGS cells.幽门螺杆菌CagA与α-Pix在胃上皮AGS细胞中的相互作用。
Ann N Y Acad Sci. 2007 Jan;1096:18-23. doi: 10.1196/annals.1397.065.
10
Alpha4 integrins are type I cAMP-dependent protein kinase-anchoring proteins.α4整合素是I型环磷酸腺苷依赖性蛋白激酶锚定蛋白。
Nat Cell Biol. 2007 Apr;9(4):415-21. doi: 10.1038/ncb1561. Epub 2007 Mar 18.
Zotero 插件