El-Zahaby Sally A, Wen Ming Ming, Abdelwahab Ibrahim A, Shahine Yasmine M, Abdelhady Sherien A, Elbatouti Gihan A
Department of Pharmaceutics and Industrial Pharmacy, PharmD Program, Egypt-Japan University of Science and Technology (E-JUST), Alexandria, Egypt.
Egypt-Japan University of Science and Technology (E-JUST), Alexandria, Egypt.
AAPS PharmSciTech. 2024 Dec 17;26(1):15. doi: 10.1208/s12249-024-03009-z.
Infections are a leading complication in patients with burns. Effective antimicrobial treatment with regenerative tissue healing is required. Utilizing components derived from plant origin such as natural oils as a sustainable and eco-friendly approach for managing disease is highly required nowadays. The aim of the current study is to assess the antibacterial and wound-healing activity of clove oil and its novel eco-friendly nanostructured liquid crystals (Eco-friendly-NLCs) formulation in treating infected burn wounds. A 2 full factorial design was used to optimize the Eco-friendly-NLCs. Clove oil and its novel nano-formulation were characterized and subjected to in vitro and in vivo assessments for their efficacy. Twenty rats were used experimentally. The optimum Eco-friendly-NLCs had 189.2 ± 1.9 nm, -22.8 ± 0.7 mV and 0.308 as values for particle size, zeta potential and polydispersity index. Transmission electron microscope images showed discrete spherical shape NLCs with no aggregations. The microbiological and pharmacological results revealed a superior efficacy regarding clove loaded Eco-friendly-NLCs in inhibiting bacterial growth (inhibition zone of 38 mm), significantly reducing inflammatory biomarker levels (p < 0.001), promoting angiogenesis and prompt wound healing. The Eco-friendly-NLCs loaded with clove oil could be considered as a promising formulation providing anti-inflammatory, anti-bacterial and wound healing effects.
感染是烧伤患者的主要并发症。需要进行有效的抗菌治疗并促进组织再生愈合。如今，迫切需要利用天然油等植物源成分作为一种可持续且环保的疾病管理方法。本研究的目的是评估丁香油及其新型环保纳米结构液晶（Eco-friendly-NLCs）制剂在治疗感染性烧伤创面中的抗菌和伤口愈合活性。采用2全因子设计优化Eco-friendly-NLCs。对丁香油及其新型纳米制剂进行了表征，并对其疗效进行了体外和体内评估。实验使用了20只大鼠。最佳的Eco-friendly-NLCs的粒径、zeta电位和多分散指数分别为189.2±1.9nm、-22.8±0.7mV和0.308。透射电子显微镜图像显示离散的球形NLCs，无聚集现象。微生物学和药理学结果显示，载有丁香的Eco-friendly-NLCs在抑制细菌生长（抑菌圈为38mm）、显著降低炎症生物标志物水平（p<0.001）、促进血管生成和促进伤口愈合方面具有卓越的疗效。载有丁香油的Eco-friendly-NLCs可被认为是一种有前景的制剂，具有抗炎、抗菌和伤口愈合作用。
