Das S, Cotter F E, Gibbons B, Dhut S, Young B D
ICRF Medical Oncology Unit, St. Bartholomew's Hospital, London, U.K.
Genes Chromosomes Cancer. 1991 Jan;3(1):44-7. doi: 10.1002/gcc.2870030108.
The t(4;11)(q21;q23) has been associated with acute lymphocytic leukemia (ALL) especially in infants. The t(4;11) breakpoint on chromosome 11 is cytogenetically indistinguishable from breakpoints for other leukemia-associated translocations affecting 11q23. The molecular basis of the t(4;11) is unknown although a number of genes have been mapped to 11q23. The CD3D, G, and E genes have been positioned proximal to the 11q23 breakpoint of the 4;11 translocation while the THY1 and ETS1 genes have been mapped distal to this breakpoint. We report evidence that CD3G is within 200 kb of the 4;11 breakpoint as observed by pulsed field gel analysis. A rearrangement of the CD3G gene has been observed in a cell line derived from a patient with the t(4;11) translocation and in a hybrid cell line containing the derivative 11q chromosome derived from this cell line, using the restriction enzymes SacII and ClaI. Similar rearrangements using SacII were observed in 2 further patients with ALL and the t(4;11) translocation. No rearrangements in the same DNA were observed using ETS1, THY1, and D11S29 and a range of rare cutter restriction enzymes. CD3G thus provides a tool for the cloning and analysis of the 4;11 translocation, and poses a question of its possible involvement at long range with this translocation.
t(4;11)(q21;q23) 与急性淋巴细胞白血病（ALL）相关，尤其在婴儿中。11号染色体上的t(4;11)断点在细胞遗传学上与影响11q23的其他白血病相关易位的断点无法区分。尽管许多基因已被定位到11q23，但t(4;11)的分子基础尚不清楚。CD3D、G和E基因位于4;11易位的11q23断点近端，而THY1和ETS1基因则位于该断点远端。我们报告了通过脉冲场凝胶分析观察到CD3G在4;11断点的200 kb范围内的证据。使用限制性内切酶SacII和ClaI，在来自一名t(4;11)易位患者的细胞系以及包含该细胞系衍生的11q衍生染色体的杂交细胞系中观察到了CD3G基因的重排。在另外2名患有ALL和t(4;11)易位的患者中使用SacII观察到了类似的重排。使用ETS1、THY1和D11S29以及一系列稀有切割限制性内切酶在相同DNA中未观察到重排。因此，CD3G为4;11易位的克隆和分析提供了一种工具，并提出了其可能在远距离参与该易位的问题。
