Hightower K R, McCready J
Eye Research Institute, Oakland University, Rochester, MI.
Curr Eye Res. 1991 Apr;10(4):299-303. doi: 10.3109/02713689108996335.
The inhibitory effects of sulfhydryl reagents on Ca-ATPase activity in the rabbit lens epithelium were assessed. Test compounds used in this study were selected on their ability to cause a calcium increase in cultured lenses. Under conditions in which lenses were cultured in the presence of the test compound, epithelial Ca-ATPase was inhibited markedly by diamide, t-BHP, IAA and slightly by selenite. The findings demonstrated that hydrogen peroxide caused little inhibition of Ca-ATPase in the lens epithelium, both when the intact lens was cultured in the presence of the oxidant or when the epithelial homogenate contained peroxide during the assay of enzyme activity. The study suggests that if a thiol-modifying compound can reach Ca-ATPase or its critical SH groups, inhibition is likely.
评估了巯基试剂对兔晶状体上皮细胞中钙-ATP酶活性的抑制作用。本研究中使用的测试化合物是根据它们在培养的晶状体中引起钙增加的能力来选择的。在晶状体在测试化合物存在下培养的条件下，二酰胺、叔丁基过氧化氢、吲哚乙酸对上皮钙-ATP酶有明显抑制作用，亚硒酸盐有轻微抑制作用。研究结果表明，无论是完整晶状体在氧化剂存在下培养，还是在酶活性测定期间上皮匀浆中含有过氧化物时，过氧化氢对晶状体上皮细胞中的钙-ATP酶几乎没有抑制作用。该研究表明，如果一种硫醇修饰化合物能够作用于钙-ATP酶或其关键的巯基基团，就可能会产生抑制作用。
