Reiner G, Fischer R, Hepp S, Berge T, Köhler F, Willems H
Department of Veterinary Clinical Sciences, University of Giessen, D-35392 Giessen, Germany.
Anim Genet. 2008 Apr;39(2):163-8. doi: 10.1111/j.1365-2052.2008.01700.x. Epub 2008 Feb 23.
Differential white blood cell counts are essential diagnostic parameters in veterinary practice but knowledge on the genetic architecture controlling variability of leucocyte numbers and relationships is sparse, especially in swine. Total leucocyte numbers (Leu) and the differential leucocyte counts, i.e. the fractions of lymphocytes (Lym), polymorphonuclear leucocytes [neutrophils (Neu), eosinophils (Eos) and basophils (Bas)] and monocytes (Mon) were measured in 139 F(2) pigs from a Meishan/Pietrain family, before and after challenge with the protozoan pathogen Sarcocystis miescheriana for genome-wide quantitative trait loci (QTL) analysis. After infection, the pigs passed through three stages representing acute disease, reconvalescence and chronic disease. Nine genome-wide significant and 29 putative, single QTL controlling leucocyte traits were identified on 15 chromosomes. Because leucocyte traits varied with health and disease status, QTL influencing the leucocyte phenotypes showed specific health/disease patterns. Regions on SSC1, 8 and 12 contained QTL for baseline leucocyte traits. Other QTL regions reached control on leucocyte traits only at distinct stages of the disease model. Two-thirds of the QTL have not been described before. Single QTL explained up to 19% of the phenotypic variance in the F(2) animals. Related traits were partly under common genetic influence. Our analysis confirms that leucocyte trait variation is associated with multiple chromosomal regions.
白细胞分类计数是兽医临床诊断的重要参数，但关于控制白细胞数量变异性及其关系的遗传结构的知识却很匮乏，尤其是在猪身上。在梅山猪/皮特兰猪杂交的139头F2代猪中，用原生动物病原体米氏肉孢子虫攻毒前后，测定了全白细胞数（Leu）和白细胞分类计数，即淋巴细胞（Lym）、多形核白细胞[中性粒细胞（Neu）、嗜酸性粒细胞（Eos）和嗜碱性粒细胞（Bas）]以及单核细胞（Mon）的比例，用于全基因组数量性状位点（QTL）分析。感染后，猪经历了代表急性病、恢复期和慢性病的三个阶段。在15条染色体上鉴定出9个全基因组显著的和29个推定的控制白细胞性状的单QTL。由于白细胞性状随健康和疾病状态而变化，影响白细胞表型的QTL呈现出特定的健康/疾病模式。SSC1、8和12号染色体上的区域包含基线白细胞性状的QTL。其他QTL区域仅在疾病模型的不同阶段对白细胞性状起控制作用。三分之二的QTL以前未曾描述过。单个QTL在F2代动物中解释了高达19%的表型变异。相关性状部分受共同遗传影响。我们的分析证实，白细胞性状变异与多个染色体区域有关。
