Reiner G, Fischer R, Köhler F, Berge T, Hepp S, Willems H
Department of Veterinary Clinical Sciences, University of Giessen, D-35392 Giessen, Germany.
Anim Genet. 2009 Apr;40(2):142-8. doi: 10.1111/j.1365-2052.2008.01813.x. Epub 2008 Dec 10.
Maintaining pH and blood gases in a narrow range is essential to sustain normal biochemical reactions. Decreased oxygenation, poor tissue perfusion, disturbance to CO(2) expiration, and shortage of HCO(3)(-) can lead to metabolic acidosis. This is a common situation in swine, and originates from a broad range of medical conditions. pH and blood gases appear to be under genetic control, and populations with physiological traits closer to the pathological thresholds may be more susceptible to developing pathological conditions. However, little is known about the genetic basis of such traits. We have therefore estimated phenotypic and genetic variability and identified quantitative trait loci (QTL) for pH and blood gases in blood samples from 139 F(2) pigs from the Meishan/Pietrain family. Samples were taken before and after challenge with Sarcocystis miescheriana, a protozoan parasite of muscle. Twenty-seven QTL influencing pH and blood gases were identified on nine chromosomes. Five of the QTL were significant on a genome-wide level; 22 QTL were significant on a chromosome-wide level. QTL for pH-associated traits have been mapped to SSC3, 18 and X. QTL associated with CO(2) have been detected on SSC6, 7, 8 and 9, and QTL associated with O(2) on SSC2 and SSC8. QTL showed specific health/disease patterns that were related to the physiological state of the pigs from day 0, to acute disease (day 14), convalescence (day 28) and chronic disease (day 42). The results demonstrate that pH and blood gases are influenced by multiple chromosomal areas, each with relatively small effects.
将pH值和血气维持在较窄范围内对于维持正常的生化反应至关重要。氧合减少、组织灌注不良、二氧化碳呼出紊乱以及碳酸氢根离子（HCO₃⁻）短缺可导致代谢性酸中毒。这在猪中是一种常见情况，且源于多种医疗状况。pH值和血气似乎受遗传控制，具有更接近病理阈值生理特征的群体可能更易患病理状况。然而，对于此类性状的遗传基础知之甚少。因此，我们估计了139头梅山/皮特兰家系F₂代猪血液样本中pH值和血气的表型及遗传变异性，并鉴定了数量性状位点（QTL）。样本在受到肌肉原虫寄生虫米氏肉孢子虫攻击前后采集。在九条染色体上鉴定出27个影响pH值和血气的QTL。其中5个QTL在全基因组水平上具有显著性；22个QTL在染色体水平上具有显著性。与pH相关性状的QTL已定位到猪的3号、18号和X染色体。与二氧化碳相关的QTL已在6号、7号、8号和9号染色体上检测到，与氧气相关的QTL在2号和8号染色体上。QTL呈现出与猪从第0天的生理状态、急性疾病（第14天）、恢复期（第28天）和慢性疾病（第42天）相关的特定健康/疾病模式。结果表明，pH值和血气受多个染色体区域影响，每个区域的影响相对较小。
