Joiner Andrew, Schwarz Annika, Philpotts Carole J, Cox Trevor F, Huber Karin, Hannig Matthias
Unilever Oral Care, Quarry Road East, Bebington, Wirral CH63 3JW, United Kingdom.
J Dent. 2008 May;36(5):360-8. doi: 10.1016/j.jdent.2008.01.010. Epub 2008 Mar 4.
To determine the protective nature of pellicle towards toothpaste abrasion.
The enamel region of human enamel-dentine blocks was indented with a Knoop diamond and the profile across the enamel-dentine junction was measured. Blocks were either exposed to deionised water or placed onto intra-oral appliances and worn in the mouth to produce in situ pellicles. This was followed by a 10-day period of tooth brushing experiments. Each day, specimens were brushed with a slurry of either Toothpaste A (RDA=90) or Toothpaste B (RDA=204) for 25 cycles (10s) on a brushing machine. This was repeated three times per day for a total of 750 brushing cycles. Between brushing cycles specimens were returned to water or in situ. The geometry of the Knoop indents and the enamel-dentine profile were re-measured and the enamel and dentine wear calculated. Specimens were also prepared for TEM analyses.
The mean enamel wear (microm) for Toothpastes A and B (water) was 0.23 and 0.06, and for Toothpastes A and B (in situ) was 0.03 and 0.08, respectively. The mean dentine wear (microm) for Toothpastes A and B (water) was 5.08 and 6.03, and for Toothpastes A and B (in situ) was 1.94 and 1.70, respectively. For Toothpaste A, the presence of in situ pellicle significantly (p<0.05) reduced enamel and dentine wear compared to water and for Toothpaste B, dentine wear was significantly reduced compared to water. After tooth brushing, residues of the in situ pellicle layer could be detected on enamel and dentine surfaces by TEM analysis.
The study has demonstrated for the first time that the presence of pellicle can significantly reduce toothpaste abrasion.
确定牙菌斑对牙膏磨损的保护作用。
用人牙釉质-牙本质块的釉质区域用努氏金刚石压痕，并测量釉质-牙本质交界处的轮廓。将牙块暴露于去离子水中或放置在口腔内装置上并在口腔中佩戴以产生原位牙菌斑。随后进行为期10天的刷牙实验。每天，将标本在刷牙机上用牙膏A（相对磨损值=90）或牙膏B（相对磨损值=204）的浆液刷25个循环（10秒）。每天重复三次，总共750个刷牙循环。在刷牙循环之间，将标本放回水中或原位。重新测量努氏压痕的几何形状和釉质-牙本质轮廓，并计算釉质和牙本质磨损。还制备了标本用于透射电子显微镜分析。
牙膏A和B（水）的平均釉质磨损（微米）分别为0.23和0.06，牙膏A和B（原位）的平均釉质磨损分别为0.03和0.08。牙膏A和B（水）的平均牙本质磨损（微米）分别为5.08和6.03，牙膏A和B（原位）的平均牙本质磨损分别为1.94和1.70。对于牙膏A，与水相比，原位牙菌斑的存在显著（p<0.05）减少了釉质和牙本质磨损，对于牙膏B，与水相比，牙本质磨损显著减少。刷牙后，通过透射电子显微镜分析可在釉质和牙本质表面检测到原位牙菌斑层的残留物。
该研究首次证明牙菌斑的存在可显著减少牙膏磨损。
