Salet C, Moreno G, Morlière P, Santus R
Laboratoire de Biophysique, INSERM U 201 et CNRS URA 481, Muséum National d'Histoire Naturelle, Paris, France.
Arch Dermatol Res. 1991;283(3):186-90. doi: 10.1007/BF00372060.
Isolated mitochondria were used to determine what causes anthralin inhibition of oxidative phosphorylation. In good agreement with other results, the rate of oxygen consumption was not modified by anthralin when mitochondria were first uncoupled with FCCP, suggesting that only the last steps of the process leading to ATP phosphorylation are implicated. No effects were found at the level of the ATPase and the Pi carrier in contrast with a competitive inhibition of the ADP/ATP translocator. These experiments suggest an atractyloside-like effect to explain the action of anthralin on mitochondria.
分离出的线粒体被用于确定蒽林抑制氧化磷酸化的原因。与其他结果高度一致的是，当线粒体先用羰基氰化物-对三氟甲氧基苯腙（FCCP）解偶联时，蒽林不会改变氧气消耗速率，这表明只有导致ATP磷酸化过程的最后步骤受到影响。与ADP/ATP转位酶的竞争性抑制相反，在ATP酶和磷酸载体水平未发现影响。这些实验表明存在一种类似苍术苷的效应来解释蒽林对线粒体的作用。
