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aro mutations in Salmonella enterica cause defects in cell wall and outer membrane integrity.肠炎沙门氏菌中的aro突变会导致细胞壁和外膜完整性出现缺陷。
J Bacteriol. 2008 May;190(9):3155-60. doi: 10.1128/JB.00053-08. Epub 2008 Feb 29.
2
aroA and aroD mutations influence biofilm formation in Salmonella Enteritidis.aroA和aroD突变影响肠炎沙门氏菌中的生物膜形成。
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J Med Microbiol. 2003 Feb;52(Pt 2):141-9. doi: 10.1099/jmm.0.04958-0.
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Genetic basis of multidrug resistance in Salmonella enterica serovars Enteritidis and Typhimurium isolated from diarrheic calves in Egypt.从埃及腹泻犊牛分离出的肠炎沙门氏菌肠炎血清型和鼠伤寒血清型多重耐药性的遗传基础。
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Antimicrobial resistance in Salmonella enterica serovars Enteritidis and Typhimurium isolated from animals in Korea: comparison of phenotypic and genotypic resistance characterization.从韩国动物中分离出的肠炎沙门氏菌肠炎血清型和鼠伤寒血清型的抗菌药物耐药性：表型和基因型耐药特征比较
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Construction and characterization of vaccine strains of Salmonella harboring mutations in two different aro genes.携带两个不同aro基因突变的沙门氏菌疫苗株的构建与特性分析
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Comparison of abilities of Salmonella enterica serovar typhimurium aroA aroD and aroA htrA mutants to act as live vectors.肠炎沙门氏菌鼠伤寒血清型aroA aroD突变体和aroA htrA突变体作为活载体的能力比较。
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本文引用的文献

1
Expression of the Yersinia enterocolitica pYV-encoded type III secretion system is modulated by lipopolysaccharide O-antigen status.小肠结肠炎耶尔森菌pYV编码的III型分泌系统的表达受脂多糖O抗原状态的调节。
Infect Immun. 2007 Mar;75(3):1512-6. doi: 10.1128/IAI.00942-06. Epub 2006 Dec 18.
2
Attenuated aroA Salmonella enterica serovar Typhimurium does not induce inflammatory response and early protection of gnotobiotic pigs against parental virulent LT2 strain.
Vaccine. 2006 May 15;24(20):4285-9. doi: 10.1016/j.vaccine.2006.02.054. Epub 2006 Mar 20.
3
Protection of gnotobiotic pigs against Salmonella enterica serotype Typhimurium by rough mutant of the same serotype is accompanied by the change of local and systemic cytokine response.同血清型粗糙突变株对无菌猪抵抗肠炎沙门氏菌鼠伤寒血清型的保护作用伴随着局部和全身细胞因子反应的变化。
Vet Immunol Immunopathol. 2005 Feb 10;103(3-4):155-61. doi: 10.1016/j.vetimm.2004.09.001.
4
Growth, virulence, and immunogenicity of Listeria monocytogenes aro mutants.单核细胞增生李斯特菌aro突变体的生长、毒力及免疫原性
Infect Immun. 2004 Oct;72(10):5622-9. doi: 10.1128/IAI.72.10.5622-5629.2004.
5
The ferritin-like Dps protein is required for Salmonella enterica serovar Typhimurium oxidative stress resistance and virulence.铁蛋白样Dps蛋白是鼠伤寒沙门氏菌抵抗氧化应激和致病力所必需的。
Infect Immun. 2004 Feb;72(2):1155-8. doi: 10.1128/IAI.72.2.1155-1158.2004.
6
Intestinal colonisation-inhibition and virulence of Salmonella phoP, rpoS and ompC deletion mutants in chickens.鸡体内鼠伤寒沙门氏菌phoP、rpoS和ompC缺失突变体的肠道定殖抑制及毒力
Vet Microbiol. 2004 Jan 14;98(1):37-43. doi: 10.1016/j.vetmic.2003.10.019.
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A new view of the mechanisms of UDP-N-acetylglucosamine enolpyruvyl transferase (MurA) and 5-enolpyruvylshikimate-3-phosphate synthase (AroA) derived from X-ray structures of their tetrahedral reaction intermediate states.基于UDP-N-乙酰葡糖胺烯醇丙酮酸转移酶（MurA）和5-烯醇丙酮酸莽草酸-3-磷酸合酶（AroA）四面体反应中间态的X射线结构，对其作用机制的新见解。
J Biol Chem. 2003 Dec 5;278(49):49215-22. doi: 10.1074/jbc.M309741200. Epub 2003 Sep 16.
8
Identification of Salmonella enterica serovar Typhimurium genes associated with growth suppression in stationary-phase nutrient broth cultures and in the chicken intestine.鉴定肠炎沙门氏菌鼠伤寒血清型中与在稳定期营养肉汤培养物和鸡肠道中生长抑制相关的基因。
Arch Microbiol. 2002 Dec;178(6):411-20. doi: 10.1007/s00203-002-0458-7. Epub 2002 Sep 19.
9
Significance analysis of microarrays applied to the ionizing radiation response.应用于电离辐射反应的微阵列显著性分析。
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10
Flow cytometry characterisation of Salmonella typhimurium mutants defective in proton translocating proteins and stationary-phase growth phenotype.鼠伤寒沙门氏菌质子转运蛋白缺陷突变体的流式细胞术表征及稳定期生长表型
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肠炎沙门氏菌中的aro突变会导致细胞壁和外膜完整性出现缺陷。

aro mutations in Salmonella enterica cause defects in cell wall and outer membrane integrity.

作者信息

Sebkova Alena, Karasova Daniela, Crhanova Magdalena, Budinska Eva, Rychlik Ivan

机构信息

Veterinary Research Institute, Hudcova 70, 621 00 Brno, Czech Republic.

出版信息

J Bacteriol. 2008 May;190(9):3155-60. doi: 10.1128/JB.00053-08. Epub 2008 Feb 29.

DOI:10.1128/JB.00053-08
PMID:18310348
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2347392/
Abstract

In this study we characterized aro mutants of Salmonella enterica serovars Enteritidis and Typhimurium, which are frequently used as live oral vaccines. We found that the aroA, aroD, and aroC mutants were sensitive to blood serum, albumen, EDTA, and ovotransferrin, and this defect could be complemented by an appropriate aro gene cloned in a plasmid. Subsequent microarray analysis of gene expression in the aroD mutant in serovar Typhimurium indicated that the reason for this sensitivity might be the upregulation of murA. To confirm this, we artificially overexpressed murA from a multicopy plasmid, and this overexpression caused sensitivity of the strain to albumen and EDTA but not to serum and ovotransferrin. We concluded that attenuation of aro mutants is caused not only by their inability to synthesize aromatic metabolites but also by their defect in cell wall and outer membrane functions associated with decreased resistance to components of innate immune response.

摘要

在本研究中，我们对肠炎沙门氏菌和鼠伤寒沙门氏菌的aro突变体进行了特性分析，这两种菌常被用作口服活疫苗。我们发现aroA、aroD和aroC突变体对血清、白蛋白、EDTA和转铁蛋白敏感，且这种缺陷可通过克隆到质粒中的合适aro基因得到互补。随后对鼠伤寒沙门氏菌aroD突变体的基因表达进行微阵列分析表明，这种敏感性的原因可能是murA的上调。为证实这一点，我们从多拷贝质粒中人工过表达murA，这种过表达导致菌株对白蛋白和EDTA敏感，但对血清和转铁蛋白不敏感。我们得出结论，aro突变体的减毒不仅是由于它们无法合成芳香族代谢产物，还由于它们在细胞壁和外膜功能方面存在缺陷，导致对先天免疫反应成分的抵抗力下降。