• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

核基质相关蛋白表达的变化与细胞转化相关。

Change in the expression of a nuclear matrix-associated protein is correlated with cellular transformation.

作者信息

Brancolini C, Schneider C

机构信息

International Center for Genetic Engineering and Biotechnology, Trieste, Italy.

出版信息

Proc Natl Acad Sci U S A. 1991 Aug 15;88(16):6936-40. doi: 10.1073/pnas.88.16.6936.

DOI:10.1073/pnas.88.16.6936
PMID:1831265
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC52208/
Abstract

We have identified a monoclonal antibody that recognizes a nuclear matrix-associated protein in NIH 3T3 cells. The immunofluorescence pattern consists predominantly of bright nuclear granule clusters distributed throughout the nucleoplasm, with the exclusion of nucleoli. It recognizes a protein of 190 kDa that is down-regulated to various degrees in a panel of single-oncogene-transformed NIH 3T3 cells. Its localization is similar, but not identical, to the spliceosomal speckles. p190 shows a coordinate expression during the growth cycle of nontransformed NIH 3T3 cells; it is synthesized at the highest level under growth arrest conditions. It is expressed in adult mouse brain and is also present in human IMR-90 fibroblasts.

摘要

我们鉴定出一种单克隆抗体，它能识别NIH 3T3细胞中一种与核基质相关的蛋白质。免疫荧光模式主要由分布于整个核质中的明亮核颗粒簇组成，核仁除外。它识别一种190 kDa的蛋白质，在一组单癌基因转化的NIH 3T3细胞中该蛋白质会不同程度地下调。其定位与剪接体斑点相似，但不完全相同。p190在未转化的NIH 3T3细胞的生长周期中呈现协同表达；在生长停滞条件下合成水平最高。它在成年小鼠脑中表达，也存在于人类IMR-90成纤维细胞中。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0058/52208/824671e4a1ef/pnas01066-0050-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0058/52208/396b8cb226fe/pnas01066-0048-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0058/52208/c6b9a3a9d08b/pnas01066-0048-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0058/52208/7cddc6791aa5/pnas01066-0048-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0058/52208/2a3ed45b9766/pnas01066-0049-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0058/52208/fad17f448072/pnas01066-0049-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0058/52208/bd60af07f491/pnas01066-0049-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0058/52208/824671e4a1ef/pnas01066-0050-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0058/52208/396b8cb226fe/pnas01066-0048-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0058/52208/c6b9a3a9d08b/pnas01066-0048-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0058/52208/7cddc6791aa5/pnas01066-0048-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0058/52208/2a3ed45b9766/pnas01066-0049-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0058/52208/fad17f448072/pnas01066-0049-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0058/52208/bd60af07f491/pnas01066-0049-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0058/52208/824671e4a1ef/pnas01066-0050-a.jpg

相似文献

1
Change in the expression of a nuclear matrix-associated protein is correlated with cellular transformation.核基质相关蛋白表达的变化与细胞转化相关。
Proc Natl Acad Sci U S A. 1991 Aug 15;88(16):6936-40. doi: 10.1073/pnas.88.16.6936.
2
Colocalization of a high molecular mass phosphoprotein of the nuclear matrix (p255) with spliceosomes.核基质的一种高分子量磷蛋白（p255）与剪接体的共定位。
J Cell Sci. 1995 May;108 ( Pt 5):1873-82. doi: 10.1242/jcs.108.5.1873.
3
In vitro heat exposure induces a redistribution of nuclear matrix proteins in human K562 erythroleukemia cells.体外热暴露诱导人K562红白血病细胞核基质蛋白重新分布。
Exp Cell Res. 1994 Jul;213(1):275-85. doi: 10.1006/excr.1994.1199.
4
Association of nuclear matrix antigens with exon-containing splicing complexes.核基质抗原与含外显子剪接复合体的关联。
J Cell Biol. 1994 Nov;127(3):593-607. doi: 10.1083/jcb.127.3.593.
5
Heat-induced stabilization of the nuclear matrix: a morphological and biochemical analysis in murine erythroleukemia cells.热诱导的核基质稳定化：小鼠红白血病细胞的形态学和生化分析
Exp Cell Res. 1991 Oct;196(2):216-25. doi: 10.1016/0014-4827(91)90254-r.
6
Nuclear matrins: identification of the major nuclear matrix proteins.核基质蛋白：主要核基质蛋白的鉴定
Proc Natl Acad Sci U S A. 1991 Nov 15;88(22):10312-6. doi: 10.1073/pnas.88.22.10312.
7
Two hnRNP-associated proteins share common structural features with the adenoviral 72-kDa protein.两种与hnRNP相关的蛋白质与腺病毒72 kDa蛋白具有共同的结构特征。
Exp Cell Res. 1995 Jan;216(1):1-12. doi: 10.1006/excr.1995.1001.
8
Heat shock-induced redistribution of a 160-kDa nuclear matrix protein.热休克诱导的一种160 kDa核基质蛋白的重新分布。
Exp Cell Res. 1992 Oct;202(2):243-51. doi: 10.1016/0014-4827(92)90071-f.
9
Intranuclear localization of a new snRNP-related antigen.一种新的与小核核糖核蛋白相关抗原的核内定位
Exp Cell Res. 1991 Dec;197(2):307-13. doi: 10.1016/0014-4827(91)90437-y.
10
Fractionation of HeLa cell nuclear extracts reveals minor small nuclear ribonucleoprotein particles.对海拉细胞核提取物进行分级分离可揭示出少量小核核糖核蛋白颗粒。
Proc Natl Acad Sci U S A. 1987 Dec;84(23):8408-12. doi: 10.1073/pnas.84.23.8408.

引用本文的文献

1
Nuclear matrix proteins in human colon cancer.人类结肠癌中的核基质蛋白
Proc Natl Acad Sci U S A. 1994 Mar 1;91(5):1913-6. doi: 10.1073/pnas.91.5.1913.
2
Phosphorylation of the growth arrest-specific protein Gas2 is coupled to actin rearrangements during Go-->G1 transition in NIH 3T3 cells.在NIH 3T3细胞从G0期向G1期转变过程中，生长停滞特异性蛋白Gas2的磷酸化与肌动蛋白重排相关联。
J Cell Biol. 1994 Mar;124(5):743-56. doi: 10.1083/jcb.124.5.743.
3
The nuclear matrix phosphoprotein p255 associates with splicing complexes as part of the [U4/U6.U5] tri-snRNP particle.

本文引用的文献

1
Variations in expression of mutant beta actin accompanying incremental increases in human fibroblast tumorigenicity.随着人类成纤维细胞瘤形成能力的逐渐增强，突变型β-肌动蛋白表达的变化
Cell. 1982 Feb;28(2):259-68. doi: 10.1016/0092-8674(82)90344-0.
2
Monoclonal antibodies to nucleic acid-containing cellular constituents: probes for molecular biology and autoimmune disease.针对含核酸细胞成分的单克隆抗体：分子生物学和自身免疫性疾病的探针
Proc Natl Acad Sci U S A. 1981 May;78(5):2737-41. doi: 10.1073/pnas.78.5.2737.
3
Nuclear matrix and hnRNP share a common structural constituent associated with premessenger RNA.
核基质磷蛋白p255作为[U4/U6.U5]三小核核糖核蛋白颗粒的一部分与剪接复合体相关联。
Nucleic Acids Res. 1995 Aug 25;23(16):3206-13. doi: 10.1093/nar/23.16.3206.
4
The effect of sodium tetrathionate stabilization on the distribution of three nuclear matrix proteins in human K562 erythroleukemia cells.连四硫酸钠稳定作用对人K562红白血病细胞核基质中三种蛋白质分布的影响
Histochem Cell Biol. 1995 Jul;104(1):29-36. doi: 10.1007/BF01464783.
5
Gas2, a growth arrest-specific protein, is a component of the microfilament network system.Gas2是一种生长停滞特异性蛋白，是微丝网络系统的一个组成部分。
J Cell Biol. 1992 Jun;117(6):1251-61. doi: 10.1083/jcb.117.6.1251.
核基质和不均一核糖核蛋白体共享一种与信使前体RNA相关的共同结构成分。
EMBO J. 1983;2(6):953-60. doi: 10.1002/j.1460-2075.1983.tb01527.x.
4
Differential expression of tropomyosin forms in the microfilaments isolated from normal and transformed rat cultured cells.原肌球蛋白形式在从正常和转化的大鼠培养细胞中分离出的微丝中的差异表达。
J Biol Chem. 1983 Nov 25;258(22):13954-64.
5
5'-Terminal caps of snRNAs are reactive with antibodies specific for 2,2,7-trimethylguanosine in whole cells and nuclear matrices. Double-label immunofluorescent studies with anti-m3G antibodies and with anti-RNP and anti-Sm autoantibodies.核内小RNA的5'-末端帽在全细胞和核基质中与针对2,2,7-三甲基鸟苷的特异性抗体发生反应。使用抗m3G抗体以及抗核糖核蛋白和抗Sm自身抗体进行双标记免疫荧光研究。
Exp Cell Res. 1984 Oct;154(2):548-60. doi: 10.1016/0014-4827(84)90179-4.
6
Stimulation of 3T3 cells induces transcription of the c-fos proto-oncogene.对3T3细胞的刺激会诱导原癌基因c-fos的转录。
Nature. 1984;311(5985):433-8. doi: 10.1038/311433a0.
7
Gene gating: a hypothesis.基因门控：一种假说。
Proc Natl Acad Sci U S A. 1985 Dec;82(24):8527-9. doi: 10.1073/pnas.82.24.8527.
8
Genes specifically expressed at growth arrest of mammalian cells.在哺乳动物细胞生长停滞时特异性表达的基因。
Cell. 1988 Sep 9;54(6):787-93. doi: 10.1016/s0092-8674(88)91065-3.
9
Differential influence of adjacent normal cells on the proliferation of mammalian cells transformed by the viral oncogenes myc, ras and src.相邻正常细胞对由病毒癌基因myc、ras和src转化的哺乳动物细胞增殖的差异影响。
Oncogene. 1988 May;2(5):509-14.
10
The distribution of two hnRNP-associated proteins defined by a monoclonal antibody is altered in heat-shocked HeLa cells.由单克隆抗体所定义的两种异质核糖核蛋白相关蛋白的分布在热休克处理的海拉细胞中发生了改变。
Exp Cell Res. 1988 Mar;175(1):109-24. doi: 10.1016/0014-4827(88)90259-5.