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对海拉细胞核提取物进行分级分离可揭示出少量小核核糖核蛋白颗粒。

Fractionation of HeLa cell nuclear extracts reveals minor small nuclear ribonucleoprotein particles.

作者信息

Krämer A

机构信息

Institute of Cell and Tumor Biology, German Cancer Research Center, Heidelberg, Federal Republic of Germany.

出版信息

Proc Natl Acad Sci U S A. 1987 Dec;84(23):8408-12. doi: 10.1073/pnas.84.23.8408.

DOI:10.1073/pnas.84.23.8408
PMID:2960976
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC299552/
Abstract

Upon chromatographic fractionation of HeLa cell nuclear extracts, small RNAs of 145 and 66/65 nucleotides, respectively, were detected that are distinct from the abundant small RNAs present in the extract. These RNAs are precipitated by antibodies directed against the trimethylguanosine cap structure, characteristic for small nuclear RNAs (snRNAs) of the U type. The RNAs of 145 and 66/65 nucleotides appear to be associated with at least one of the proteins common to the major small nuclear ribonucleoprotein particles U1 to U6, since they are specifically bound by anti-Sm antibodies. These criteria characterize the RNAs that are 145 and 66/65 nucleotides in length as U-type snRNAs. Upon gel filtration, the RNAs are found within particles of molecular weights approximately equal to 150,000 and 115,000, respectively. The RNA of 145 nucleotides represents a different minor snRNA, designated U11, whereas the RNA of 66/65 nucleotides may correspond to either mammalian U7 or U10 RNA.

摘要

对HeLa细胞核提取物进行色谱分级分离时，分别检测到了145个核苷酸和66/65个核苷酸的小RNA，它们与提取物中存在的丰富小RNA不同。这些RNA可被针对三甲基鸟苷帽结构的抗体沉淀，该结构是U型小核RNA（snRNA）的特征。145个核苷酸和66/65个核苷酸的RNA似乎与主要小核核糖核蛋白颗粒U1至U6共有的至少一种蛋白质相关，因为它们能被抗Sm抗体特异性结合。这些标准将长度为145个核苷酸和66/65个核苷酸的RNA鉴定为U型snRNA。通过凝胶过滤发现，这些RNA分别存在于分子量约为150,000和115,000的颗粒中。145个核苷酸的RNA代表一种不同的次要snRNA，命名为U11，而66/65个核苷酸的RNA可能对应于哺乳动物的U7或U10 RNA。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/52e9/299552/b541bc4a8733/pnas00338-0246-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/52e9/299552/ef6756d647cc/pnas00338-0244-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/52e9/299552/64e688699601/pnas00338-0244-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/52e9/299552/b1ccd427637a/pnas00338-0245-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/52e9/299552/e23a9b58f3d4/pnas00338-0245-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/52e9/299552/e2bb25072b76/pnas00338-0246-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/52e9/299552/b541bc4a8733/pnas00338-0246-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/52e9/299552/ef6756d647cc/pnas00338-0244-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/52e9/299552/64e688699601/pnas00338-0244-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/52e9/299552/b1ccd427637a/pnas00338-0245-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/52e9/299552/e23a9b58f3d4/pnas00338-0245-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/52e9/299552/e2bb25072b76/pnas00338-0246-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/52e9/299552/b541bc4a8733/pnas00338-0246-b.jpg

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本文引用的文献

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通过与纯化的人源U1、U2、U4/U6和U5小核核糖核蛋白互补进行前体mRNA剪接。
Nucleic Acids Res. 1988 Oct 25;16(20):9415-29. doi: 10.1093/nar/16.20.9415.
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