Prudent Michel, Girault Hubert H
Laboratoire d'Electrochimie Physique et Analytique, Ecole Polytechnique Fédérale de Lausanne, Lausanne, Switzerland.
J Am Soc Mass Spectrom. 2008 Apr;19(4):560-8. doi: 10.1016/j.jasms.2008.01.008. Epub 2008 Jan 31.
The interaction of copper ions with peptides was investigated by electrospray mass spectrometry. Two electrospray micro-emitters were compared, the first one with a platinum electrode using a copper(II) electrolyte solution containing a peptide sample, and the second one with a sacrificial copper anode in a water/methanol solution containing only a peptide (i.e., angiotensin III, bradykinin, or Leu-enkephalin). The former yielded mainly Cu(2+) complexes either with histidine residues or with the peptide backbone (Cu(+) complexes can be also formed due to gas-phase reactions), whereas the latter can generate a mixture of both Cu(+) and Cu(2+) aqueous complexes that yield different complexation patterns. This study shows that electrospray emitters with soluble copper anodes enable the study of Cu(I)-peptide complexes in solution.
通过电喷雾质谱法研究了铜离子与肽的相互作用。比较了两种电喷雾微发射器，第一种使用铂电极，在含有肽样品的铜（II）电解质溶液中；第二种在仅含有肽（即血管紧张素III、缓激肽或亮氨酸脑啡肽）的水/甲醇溶液中使用牺牲铜阳极。前者主要产生与组氨酸残基或肽主链形成的Cu(2+)络合物（由于气相反应也可形成Cu(+)络合物），而后者可生成Cu(+)和Cu(2+)水相络合物的混合物，产生不同的络合模式。这项研究表明，带有可溶性铜阳极的电喷雾发射器能够研究溶液中的Cu(I)-肽络合物。
