酵母中线粒体的荧光成像

Fluorescence imaging of mitochondria in yeast.

作者信息

Swayne Theresa C, Gay Anna C, Pon Liza A

机构信息

Department of Anatomy and Cell Biology, College of Physicians and Surgeons, Columbia University, New York, NY, USA.

出版信息

Methods Mol Biol. 2007;372:433-59. doi: 10.1007/978-1-59745-365-3_31.

DOI:10.1007/978-1-59745-365-3_31

PMID:18314744

Abstract

The budding yeast Saccharomyces cerevisiae has many advantages as a model system, but until recently high-resolution microscopy was not often attempted in this organism. Its small size, rounded shape, and rigid cell wall were obstacles to exploring the cell biology of this model eukaryote. However, it is now feasible for laboratories to acquire and analyze high-resolution, multidimensional images of yeast cell biology, including the mitochondria. As a result, imaging of yeast has emerged as an important tool in eukaryotic cell biology. This chapter describes labeling methods and optical approaches for visualizing yeast mitochondria using fluorescence microscopy.

摘要

出芽酵母酿酒酵母作为一种模式系统有许多优点，但直到最近，很少有人尝试在这种生物体中进行高分辨率显微镜观察。它的小尺寸、圆形形状和坚硬的细胞壁是探索这种模式真核生物细胞生物学的障碍。然而，现在实验室获取和分析酵母细胞生物学的高分辨率、多维图像（包括线粒体）已成为可行之事。因此，酵母成像已成为真核细胞生物学中的一种重要工具。本章描述了使用荧光显微镜观察酵母线粒体的标记方法和光学方法。