酿酒酵母作为研究线粒体生物学的模式生物：一般考量与基本程序

Saccharomyces cerevisiae as a model organism to study mitochondrial biology: general considerations and basic procedures.

作者信息

Altmann Katrin, Dürr Mark, Westermann Benedikt

机构信息

Institut für Zellbiologie, Universität Bayreuth, Germany.

出版信息

Methods Mol Biol. 2007;372:81-90. doi: 10.1007/978-1-59745-365-3_6.

DOI:10.1007/978-1-59745-365-3_6

PMID:18314719

Abstract

Budding yeast Saccharomyces cerevisiae is widely used to study mitochondrial biogenesis and function. We review some basic properties that make yeast an ideal model organism to investigate various aspects of mitochondrial biology. We discuss genetic features of commonly used yeast strains that are important for mitochondrial studies. Furthermore, this chapter provides protocols describing yeast culture conditions and procedures for isolation and purification of mitochondria.

摘要

出芽酵母酿酒酵母被广泛用于研究线粒体的生物发生和功能。我们回顾了一些使酵母成为研究线粒体生物学各个方面的理想模式生物的基本特性。我们讨论了常用于线粒体研究的酵母菌株的遗传特征。此外，本章提供了描述酵母培养条件以及线粒体分离和纯化程序的实验方案。