Soler Marçal, Serra Olga, Molinas Marisa, García-Berthou Emili, Caritat Antònia, Figueras Mercè
Laboratori del Suro, Department of Biology, Universitat de Girona, Campus Montilivi s/n, Girona, Spain.
Tree Physiol. 2008 May;28(5):743-51. doi: 10.1093/treephys/28.5.743.
The molecular processes underlying cork biosynthesis and differentiation are mostly unknown. Recently, a list of candidate genes for cork biosynthesis and regulation was made available opening new possibilities for molecular studies in cork oak (Quercus suber L.). Based on this list, we analyzed the seasonal variation in mRNA abundance in cork tissue of selected genes by real time reverse-transcriptase polymerase chain reaction (RT-PCR). Relative transcript abundance was evaluated by principal component analysis and genes were clustered in several functional subgroups. Structural genes of suberin pathways such as CYP86A1, GPAT and HCBT, and regulatory genes of the NAM and WRKY families showed highest transcript accumulation in June, a crucial month for cork development. Other cork structural genes, such as FAT and F5H, were significantly correlated with temperature and relative humidity. The stress genes HSP17.4 and ANN were strongly positively correlated to temperature, in accord with their protective role.
软木塞生物合成和分化背后的分子过程大多未知。最近，一份软木塞生物合成和调控的候选基因列表公布，为栓皮栎（Quercus suber L.）的分子研究开辟了新的可能性。基于此列表，我们通过实时逆转录聚合酶链反应（RT-PCR）分析了选定基因在软木组织中mRNA丰度的季节性变化。通过主成分分析评估相对转录本丰度，并将基因聚类到几个功能亚组中。木栓质途径的结构基因，如CYP86A1、GPAT和HCBT，以及NAM和WRKY家族的调控基因在6月显示出最高的转录本积累，这是软木塞发育的关键月份。其他软木结构基因，如FAT和F5H，与温度和相对湿度显著相关。应激基因HSP17.4和ANN与温度呈强烈正相关，这与其保护作用一致。
