Rudman D
Neuroendocrinology. 1976;20(3):235-42. doi: 10.1159/000122487.
Rabbits were anesthetized with urethane and were given intracisternal injections of the following substances: adrenocorticotropin, beta-melanocyte stimulating hormone, choroid plexus peptide IIF, epinephrine, serotonin, histamine, oxytocin, lysine and a arginine vasopressins, acetylcholine and melatonin. The effects on the concentration of 3', 5' cyclic guanosine monophosphate (cGMP) in cerebrospinal fluid were then measured. Only melatonin and acetylcholine caused a significant (p less than 0.05) effect on cGMP concentration. Both agents increased the nucleotide's concentration within 30 min. Melatonin was about 1,000 times more potent than acetylcholine; the mininal effective doses were 1 mug and 1,000 mug, respectively.
用氨基甲酸乙酯麻醉兔子，并向其脑池内注射以下物质：促肾上腺皮质激素、β-黑素细胞刺激激素、脉络丛肽IIF、肾上腺素、血清素、组胺、催产素、赖氨酸和精氨酸加压素、乙酰胆碱和褪黑素。然后测量这些物质对脑脊液中3',5'-环鸟苷单磷酸（cGMP）浓度的影响。只有褪黑素和乙酰胆碱对cGMP浓度有显著影响（p<0.05）。两种药物均在30分钟内增加了核苷酸的浓度。褪黑素的效力比乙酰胆碱强约1000倍；最小有效剂量分别为1微克和1000微克。
