Ma Chunhui, Fan Mingsong, Tang Yihong, Li Zhixiong, Sun Zhaolin, Ye Guan, Huang Chenggang
Shanghai Institute of Materia Medica, Shanghai Institute for Biological Science, Chinese Academy of Sciences, Shanghai, People's Republic of China.
Biomed Chromatogr. 2008 Aug;22(8):835-50. doi: 10.1002/bmc.1000.
Huangbai-Zhimu herb-pair (HBZMHP) is a widely used Chinese traditional medicine formula in treating various diseases; however, its active components have remained unknown. In this paper, serum chemistry and combined high-performance liquid chromatography (HPLC), diode-array detection and mass-spectrometry (MS) techniques were used to study the constituents of HBZMHP extract absorbed into rat serum after oral administration. A total of nine characteristic HPLC peaks in the TIC chromatograms were identified as magnoflorine (1), menisperine (2), palmatine (3), berberine (4), timosaponin N or timosaponin E1 (5), timosaponin D (6), timosaponin BIII, anemarsaponin C or xilingsaponin B (7) timosaponin BII (8) and timosaponin AIII (9). All of the identified peaks were constituents of HBZMHP extract. The results narrow the range of active compounds to be found in HBZMHP extract, and pave the way for the follow-up action mechanism research.
黄柏-知母药对（HBZMHP）是一种广泛用于治疗各种疾病的中药配方；然而，其活性成分尚不清楚。本文采用血清化学结合高效液相色谱（HPLC）、二极管阵列检测和质谱（MS）技术，研究口服给药后大鼠血清中吸收的HBZMHP提取物的成分。总离子流色谱图中的9个特征HPLC峰分别被鉴定为木兰碱（1）、千金藤碱（2）、巴马汀（3）、小檗碱（4）、知母皂苷N或知母皂苷E1（5）、知母皂苷D（6）、知母皂苷BIII、银莲花皂苷C或细叶柴胡皂苷B（7）、知母皂苷BII（8）和知母皂苷AIII（9）。所有鉴定出的峰均为HBZMHP提取物的成分。这些结果缩小了在HBZMHP提取物中有待发现的活性化合物的范围，并为后续作用机制研究铺平了道路。
