Yacoby Iftach, Benhar Itai
Tel Aviv University, Department of Molecular Microbiology and Biotechnology, Green Building, Room 202, Ramat Aviv 69978, Israel.
Expert Opin Drug Deliv. 2008 Mar;5(3):321-9. doi: 10.1517/17425247.5.3.321.
Bacteriophages (phages) have been used for therapy of bacterial infections, for genetic research, as tools for the discovery of specific target binding proteins and for vaccine development. The aim of this article is to present advances in genetic and chemical engineering of filamentous bacteriophages that facilitated their application for therapeutic purposes. We review studies where phages were applied for in vivo imaging, as gene delivery vehicles and as drug carriers. Target specificity is based on peptides or proteins displayed on the phage coat. The cargo may be a packaged gene incorporated into the phage genome for gene delivery applications, or imaging agents or cytotoxic drugs chemically conjugated at high density onto the phage coat. We believe that the combination of those separately developed methodologies would result in clinical applications of phage-based therapeutics.
噬菌体已被用于细菌感染的治疗、基因研究、作为发现特定靶标结合蛋白的工具以及疫苗开发。本文旨在介绍丝状噬菌体基因工程和化学工程方面的进展，这些进展推动了其在治疗领域的应用。我们综述了噬菌体用于体内成像、作为基因传递载体和药物载体的研究。靶向特异性基于展示在噬菌体衣壳上的肽或蛋白质。货物可以是为基因传递应用而整合到噬菌体基因组中的包装基因，或者是高密度化学偶联到噬菌体衣壳上的成像剂或细胞毒性药物。我们相信，这些分别开发的方法的结合将导致基于噬菌体的治疗方法的临床应用。
