Lim Ju Hyoung, Baek Sang-Hoon, Lee Sung-Taik
Environmental and Molecular Microbiology Lab., Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 335 Gwahangno, Yuseong-gu, Daejeon 305-701, Republic of Korea.
Int J Syst Evol Microbiol. 2008 Mar;58(Pt 3):565-9. doi: 10.1099/ijs.0.65502-0.
A Gram-negative, motile and rod-shaped bacterium, designated HU2-65W(T), was isolated from freshwater sediment. The strain possessed ubiquinone 8 as the predominant isoprenoid quinone and contained major amounts of omega7-cis-octadecenoic acid and hexadecanoic acid in its cell envelope, which are properties shared by members of the genus Burkholderia. On the basis of 16S rRNA gene sequence similarity, strain HU2-65W(T) was most closely related to the type strain of Burkholderia xenovorans (98.4 %). The DNA G+C content of strain HU2-65W(T) was 61.2 mol%, and DNA-DNA relatedness values to type strains of closely related species were found to be much lower than 70 %, indicating that the strain represents a separate genomic species within the genus Burkholderia. Strain HU2-65W(T) was also differentiated from other species of the genus by physiological and biochemical characteristics. Consequently, strain HU2-65W(T) is considered to represent a single, novel species of the genus Burkholderia, for which the name Burkholderia sediminicola sp. nov. is proposed, with the type strain HU2-65W(T) (=KCTC 22086(T) =LMG 24238(T)).
从淡水沉积物中分离出一株革兰氏阴性、具运动性的杆状细菌，命名为HU2-65W(T)。该菌株以泛醌8作为主要类异戊二烯醌，其细胞膜中含有大量的ω7-顺式十八碳烯酸和十六烷酸，这些是伯克霍尔德氏菌属成员共有的特性。基于16S rRNA基因序列相似性，菌株HU2-65W(T)与洋葱伯克霍尔德氏菌（Burkholderia xenovorans）模式菌株的亲缘关系最为密切（98.4%）。菌株HU2-65W(T)的DNA G+C含量为61.2 mol%，与近缘物种模式菌株的DNA-DNA杂交值远低于70%，表明该菌株代表伯克霍尔德氏菌属内一个独立的基因组物种。菌株HU2-65W(T)在生理生化特征上也与该属的其他物种不同。因此，菌株HU2-65W(T)被认为代表伯克霍尔德氏菌属的一个新的单一物种，为此提出新名称伯克霍尔德氏菌属沉积物伯克霍尔德氏菌（Burkholderia sediminicola sp. nov.），模式菌株为HU2-65W(T)（=KCTC 2208(T)=LMG 24238(T)）。
