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sp. nov.,一种新型的植物相关生长促进剂。

sp. nov., a novel plant associated growth promotor.

机构信息

Molecular Systems Biology Division (MOSYS), Department of Functional and Evolutionary Ecology, University of Vienna, Djerassiplatz 1, 1030 Vienna, Austria.

Laboratory of Microbiology, Department of Biochemistry and Microbiology, Ghent University, K. L. Ledeganckstraat 35, 9000 Ghent, Belgium.

出版信息

Int J Syst Evol Microbiol. 2021 Sep;71(9). doi: 10.1099/ijsem.0.004969.

Abstract

A novel bacterium, designated strain Msb3, was recently isolated from leaves of the yam family plant (). Phylogenetic analysis based on the 16S rRNA gene sequence indicated that this strain belonged to the genus with as nearest validly named neighbour taxon (99.3 % sequence similarity towards the type strain). Earlier genome sequence analysis revealed a genome of 8.35 Mb in size with a G+C content of 62.5 mol%, which was distributed over two chromosomes and three plasmids. Here, we confirm that strain Msb3 represents a novel species. DNA-DNA hybridization and average nucleotide identity (OrthoANIu) analyses towards LB400 yielded 58.4 % dDDH and 94.5 % orthoANIu. Phenotypic and metabolic characterization revealed growth at 15 °C on tryptic soy agar, growth in the presence of 1 % NaCl and the lack of assimilation of phenylacetic acid as distinctive features. Together, these data demonstrate that strain Msb3 represents a novel species of the genus for which we propose the name sp. nov. The type strain is Msb3 (=LMG 31881, DSM 111632, CECT 30342).

摘要

一种新型细菌,被命名为 Msb3 菌株,最近从山药科植物的叶子中分离出来。基于 16S rRNA 基因序列的系统发育分析表明,该菌株属于,最接近的有效命名近缘分类群是(与 模式菌株的序列相似性为 99.3%)。早期的基因组序列分析显示,其基因组大小为 8.35 Mb,GC 含量为 62.5 mol%,分布在两个染色体和三个质粒上。在这里,我们确认 Msb3 菌株代表了一个新型的 物种。 与 LB400 的 DNA-DNA 杂交和平均核苷酸同一性(OrthoANIu)分析得出 58.4%的 dDDH 和 94.5%的 orthoANIu。表型和代谢特征分析表明,在胰蛋白胨大豆琼脂上于 15°C 生长、在 1%NaCl 存在下生长以及缺乏苯乙酸同化是其显著特征。综上所述,这些数据表明 Msb3 菌株代表了一个新型的 属的物种,我们建议将其命名为 sp. nov.。其模式菌株为 Msb3(=LMG 31881、DSM 111632、CECT 30342)。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa7c/8549267/f7dd6ae15d5c/ijsem-71-4969-g001.jpg

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