Yoon Jaewoo, Matsuo Yoshihide, Katsuta Atsuko, Jang Jae-Hyuk, Matsuda Satoru, Adachi Kyoko, Kasai Hiroaki, Yokota Akira
Institute of Molecular and Cellular Biosciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-Ku, Tokyo 113-0032, Japan.
Int J Syst Evol Microbiol. 2008 Nov;58(Pt 11):2491-500. doi: 10.1099/ijs.0.2008/000711-0.
Six Gram-negative, non-motile, rod-shaped, chemoheterotrophic bacteria designated strains YM23-227(T), 06SJR1-1(T), AK18-024(T), 05IJR53-1(T), MN1-1037(T) and MN1-1047 were isolated from various marine environments and subjected to a polyphasic taxonomic study. Preliminary analysis based on 16S rRNA gene sequences revealed that the novel isolates could be affiliated with the family Verrucomicrobiaceae of the phylum 'Verrucomicrobia'. The strains shared approximately 94-100 % sequence similarity with each other and showed less than 94 % similarity with members of the family Verrucomicrobiaceae with validly published names. The DNA-DNA relatedness between strains YM23-227(T) and 06SJR1-1(T) was less than 70 %, a value that is accepted as a phylogenetic definition of a species. The cell wall peptidoglycan of the strains contained muramic acid and meso-diaminopimelic acid. The novel isolates produced carotenoid pigments and squalene. The DNA G+C contents of the six strains were 63-65 mol%. The major menaquinone was MK-9 and iso-C(14 : 0) was the major fatty acid. Based on the evidence from the polyphasic taxonomic study, it was concluded that the six strains should be classified as representing a new genus and five novel species of the family Verrucomicrobiaceae within the phylum 'Verrucomicrobia', for which the names Haloferula rosea gen. nov., sp. nov. (type strain 06SJR1-1(T)=MBIC08340(T)=KCTC 22201(T)), Haloferula harenae sp. nov. (type strain YM23-227(T)=MBIC08299(T)=KCTC 22198(T)), Haloferula phyci sp. nov. (type strain AK18-024(T)=MBIC08341(T)=KCTC 22200(T)), Haloferula helveola sp. nov. (type strain 05IJR53-1(T)=MBIC08342(T)=KCTC 22199(T)) and Haloferula sargassicola sp. nov. (type strain MN1-1037(T)=MBIC08343(T)=KCTC 22202(T)) are proposed.
从不同海洋环境中分离出六株革兰氏阴性、无运动性、杆状、化能异养细菌，分别命名为菌株YM23 - 227(T)、06SJR1 - 1(T)、AK18 - 024(T)、05IJR53 - 1(T)、MN1 - 1037(T)和MN1 - 1047，并对其进行了多相分类研究。基于16S rRNA基因序列的初步分析表明，这些新分离菌株可归属于“疣微菌门”疣微菌科。这些菌株彼此之间的序列相似性约为94% - 100%，与已有效发表名称的疣微菌科成员的相似性低于94%。菌株YM23 - 227(T)和06SJR1 - 1(T)之间的DNA - DNA相关性低于70%，该值被认为是物种的系统发育定义。这些菌株的细胞壁肽聚糖含有胞壁酸和内消旋二氨基庚二酸。这些新分离菌株产生类胡萝卜素色素和角鲨烯。这六株菌株的DNA G + C含量为63 - 65 mol%。主要的甲基萘醌是MK - 9，主要脂肪酸是异C(14 : 0)。基于多相分类研究的证据，得出结论：这六株菌株应被分类为代表“疣微菌门”内疣微菌科的一个新属和五个新物种，为此提出新属名为Haloferula，新物种名为玫瑰色嗜盐菌（Haloferula rosea）（模式菌株06SJR1 - 1(T)=MBIC08340(T)=KCTC 22201(T)）、沙质嗜盐菌（Haloferula harenae）（模式菌株YM
