Weon Hang-Yeon, Kim Byung-Yong, Son Jung-A, Song Myung-Hee, Kwon Soon-Wo, Go Seung-Joo, Stackebrandt Erko
Applied Microbiology Division, National Institute of Agricultural Science and Technology, Rural Development Administration (RDA), Suwon 441-707, Republic of Korea.
Int J Syst Evol Microbiol. 2008 Mar;58(Pt 3):578-80. doi: 10.1099/ijs.0.64994-0.
A Gram-negative bacterium, designated strain GR15-1(T), was isolated from a field cultivated with Korean ginseng. Cells were strictly aerobic, motile with multipolar flagella and rod-shaped. The strain grew optimally at 25-28 degrees C and pH 6.0-7.0. The predominant fatty acids of strain GR15-1(T) were C(18 : 1)omega7c, C(16 : 0) and summed feature 2 (C(14 : 0) 3-OH and/or iso-C(16 : 1) I). The major isoprenoid quinone was ubiquinone 8. The G+C content of the genomic DNA was 63.9 mol%. Phylogenetic analysis showed that strain GR15-1(T) formed a phyletic cluster with Nevskia ramosa Soe1(T), with a 16S rRNA gene sequence similarity of 96.8 %. Based on phenotypic, chemotaxonomic and phylogenetic features, strain GR15-1(T) represents a novel species within the genus Nevskia, for which the name Nevskia soli sp. nov. is proposed. The type strain is GR15-1(T) (=KACC 11703(T) =DSM 19509(T)).
从种植高丽参的田间分离出一株革兰氏阴性菌，命名为GR15-1(T)菌株。细胞为严格需氧型，具多极鞭毛，呈杆状。该菌株在25 - 28℃和pH 6.0 - 7.0条件下生长最佳。GR15-1(T)菌株的主要脂肪酸为C(18 : 1)ω7c、C(16 : 0)和总和特征2（C(14 : 0) 3-OH和/或异-C(16 : 1) I）。主要类异戊二烯醌为泛醌8。基因组DNA的G+C含量为63.9 mol%。系统发育分析表明，GR15-1(T)菌株与拉莫萨涅夫斯基菌Soe1(T)形成一个系统发育簇，16S rRNA基因序列相似性为96.8%。基于表型、化学分类和系统发育特征，GR15-1(T)菌株代表涅夫斯基菌属内的一个新物种，为此提出新名称涅夫斯基土壤杆菌（Nevskia soli sp. nov.）。模式菌株为GR15-1(T)（=KACC 11703(T) =DSM 19509(T)）。
