Weon Hang-Yeon, Yoo Seung-Hee, Kim Byung-Yong, Son Jung-A, Kim Yoo-Jeong, Kwon Soon-Wo
Applied Microbiology Division, National Institute of Agricultural Science and Technology, Rural Development Administration (RDA), Suwon 441-707, Republic of Korea.
Int J Syst Evol Microbiol. 2009 Jun;59(Pt 6):1282-5. doi: 10.1099/ijs.0.004333-0.
The taxonomic status of a yellow- to light orange-coloured strain isolated from soil of a Korean ginseng field was established based on a polyphasic investigation. The novel isolate, strain GR10-1(T), was an obligately aerobic, Gram-staining-negative, non-motile, flexirubin-pigment-producing, short rod-shaped bacterium. The strain grew optimally at 28-30 degrees C, at pH 7.0 and in the presence of 0-1 % NaCl. Phylogenetic analyses based on 16S rRNA gene sequences demonstrated that the new isolate showed the highest sequence similarities with Niabella aurantiaca R2A15-11(T) (95.1 %) and Niabella soli JS13-8(T) (94.6 %). The DNA G+C content of strain GR10-1(T) was 43 mol%. It contained iso-C(15 : 1) G (36.4 %) and iso-C(15 : 0) (32.8 %) as the major fatty acids (>10 %) and MK-7 as the major isoprenoid quinone. On the basis of evidence from our polyphasic taxonomic study, it was concluded that strain GR10-1(T) should be classified within a novel species of the genus Niabella, for which the name Niabella ginsengisoli sp. nov. is proposed. The type strain is GR10-1(T) (=KACC 13021(T) =JCM 15444(T)).
基于多相分类研究，确定了从韩国人参田土壤中分离出的一株黄色至浅橙色菌株的分类地位。新分离菌株GR10-1(T)是专性需氧、革兰氏染色阴性、无运动性、产生flexirubin色素的短杆状细菌。该菌株在28-30℃、pH 7.0以及0-1% NaCl存在的条件下生长最佳。基于16S rRNA基因序列的系统发育分析表明，新分离菌株与金黄尼氏菌R2A15-11(T)（95.1%）和土壤尼氏菌JS13-8(T)（94.6%）的序列相似性最高。菌株GR10-1(T)的DNA G+C含量为43 mol%。其主要脂肪酸（>10%）为异-C(15 : 1) G（36.4%）和异-C(15 : 0)（32.8%），主要类异戊二烯醌为MK-7。基于我们多相分类研究的证据，得出结论：菌株GR10-1(T)应归类于尼氏菌属的一个新物种，为此提出名称人参土壤尼氏菌（Niabella ginsengisoli sp. nov.）。模式菌株为GR10-1(T)（=KACC 13021(T) =JCM 15444(T)）。
