Ten Leonid N, Jung Hae-Min, Im Wan-Taek, Yoo Soon-Ae, Oh Hee-Mock, Lee Sung-Taik
Department of Biology and Medicinal Science, Pai Chai University, 14 Yeonja-1-Gil, Seo-Gu, Daejeon 302-735, Republic of Korea.
Int J Syst Evol Microbiol. 2009 May;59(Pt 5):958-63. doi: 10.1099/ijs.0.002394-0.
A Gram-negative, aerobic, rod-shaped, non-spore-forming bacterial strain, designated Gsoil 068(T), was isolated from soil of a ginseng field in Pocheon Province (South Korea), and was characterized to determine its taxonomic position by using a polyphasic approach. Comparative 16S rRNA gene sequence analysis showed that strain Gsoil 068(T) belonged to the family Xanthomonadaceae, class Gammaproteobacteria, and was related most closely to Lysobacter brunescens ATCC 29482(T) and Lysobacter gummosus ATCC 29489(T) (96.1 % sequence similarity). The G+C content of the genomic DNA of strain Gsoil 068(T) was 67.0 mol%. The detection of a quinone system with ubiquinone Q-8 as the predominant component and a fatty acid profile with iso-C(15 : 0), iso-C(17 : 1)omega9c, iso-C(17 : 0) and iso-C(11 : 0) 3-OH as the major components supported the affiliation of strain Gsoil 068(T) to the genus Lysobacter. On the basis of its phenotypic properties and phylogenetic distinctiveness, strain Gsoil 068(T) is considered to represent a novel species of the genus Lysobacter, for which the name Lysobacter panaciterrae sp. nov. is proposed. The type strain is Gsoil 068(T) (=KCTC 12601(T) =DSM 17927(T)).
从韩国抱川市人参田土壤中分离出一株革兰氏阴性、需氧、杆状、不形成芽孢的细菌菌株，命名为Gsoil 068(T)，并采用多相分类法对其进行特征分析以确定其分类地位。16S rRNA基因序列比较分析表明，菌株Gsoil 068(T)属于黄单胞菌科γ-变形菌纲，与褐黄溶杆菌ATCC 29482(T)和黏液溶杆菌ATCC 29489(T)关系最为密切（序列相似性为96.1%）。菌株Gsoil 068(T)基因组DNA的G+C含量为67.0 mol%。以泛醌Q-8为主要成分的醌系统以及以异C(15 : 0)、异C(17 : 1)ω9c、异C(17 : 0)和异C(11 : 0) 3-OH为主要成分的脂肪酸谱分析结果支持菌株Gsoil 068(T)归属于溶杆菌属。基于其表型特征和系统发育独特性，菌株Gsoil 068(T)被认为代表溶杆菌属的一个新物种，为此提出新名称人参田溶杆菌（Lysobacter panaciterrae sp. nov.）。模式菌株为Gsoil 068(T)（=KCTC 12601(T) =DSM 17927(T)）。
