Slobodkina G B, Kolganova T V, Chernyh N A, Querellou J, Bonch-Osmolovskaya E A, Slobodkin A I
Winogradsky Institute of Microbiology, Russian Academy of Sciences, Prospect 60-letiya Oktyabrya 7/2, 117312 Moscow, Russia.
Int J Syst Evol Microbiol. 2009 Jun;59(Pt 6):1508-12. doi: 10.1099/ijs.0.006767-0.
A thermophilic, anaerobic, chemolithoautotrophic bacterium (designated strain SL50(T)) was isolated from a hydrothermal sample collected at the Mid-Atlantic Ridge from the deepest of the known World ocean hydrothermal fields, Ashadze field (1 degrees 58' 21'' N 4 degrees 51' 47'' W) at a depth of 4100 m. Cells of strain SL50(T) were motile, straight to bent rods with one polar flagellum, 0.5-0.6 mum in width and 3.0-3.5 mum in length. The temperature range for growth was 25-75 degrees C, with an optimum at 60 degrees C. The pH range for growth was 5.0-7.5, with an optimum at pH 6.5. Growth of strain SL50(T) was observed at NaCl concentrations ranging from 1.0 to 6.0 % (w/v) with an optimum at 2.5 % (w/v). The generation time under optimal growth conditions for strain SL50(T) was 60 min. Strain SL50(T) used molecular hydrogen, acetate, lactate, succinate, pyruvate and complex proteinaceous compounds as electron donors, and Fe(III), Mn(IV), nitrate or elemental sulfur as electron acceptors. The G+C content of the DNA of strain SL50(T) was 28.7 mol%. 16S rRNA gene sequence analysis revealed that the closest relative of strain SL50(T) was Deferribacter abyssi JR(T) (95.5 % similarity). On the basis of its physiological properties and phylogenetic analyses, the isolate is considered to represent a novel species, for which the name Deferribacter autotrophicus sp. nov. is proposed. The type strain is SL50(T) (=DSM 21529(T)=VKPM B-10097(T)). Deferribacter autotrophicus sp. nov. is the first described deep-sea bacterium capable of chemolithoautotrophic growth using molecular hydrogen as an electron donor and ferric iron as electron acceptor and CO(2) as the carbon source.
从大西洋中脊已知世界海洋最深热液区——阿沙德泽热液区（北纬1度58分21秒，西经4度51分47秒）4100米深处采集的热液样本中分离出一株嗜热、厌氧、化能无机自养细菌（命名为菌株SL50(T)）。菌株SL50(T)的细胞具运动性，为直杆状至弯杆状，有一根极生鞭毛，宽度为0.5 - 0.6微米，长度为3.0 - 3.5微米。生长温度范围为25 - 75℃，最适温度为60℃。生长pH范围为5.0 - 7.5，最适pH为6.5。在NaCl浓度为1.0%至6.0%（w/v）范围内观察到菌株SL50(T)生长，最适浓度为2.5%（w/v）。菌株SL50(T)在最佳生长条件下的代时为60分钟。菌株SL50(T)利用分子氢、乙酸盐、乳酸盐、琥珀酸盐、丙酮酸盐和复杂蛋白质化合物作为电子供体，利用Fe(III)、Mn(IV)、硝酸盐或元素硫作为电子受体。菌株SL50(T)的DNA的G + C含量为28.7 mol%。16S rRNA基因序列分析表明，菌株SL50(T)最接近的亲缘种是深渊脱铁杆菌JR(T)（相似度为95.5%）。基于其生理特性和系统发育分析，该分离株被认为代表一个新物种，为此提出新名称嗜热自养脱铁杆菌（Deferribacter autotrophicus sp. nov.）。模式菌株为SL50(T)（=DSM 21529(T)=VKPM B - 10097(T)）。嗜热自养脱铁杆菌（Deferribacter autotrophicus sp. nov.）是首次描述的能够以分子氢作为电子供体、三价铁作为电子受体和二氧化碳作为碳源进行化能无机自养生长的深海细菌。
