Callas Ney, Poveda Elpidia, Baracaldo César, Hernández Patricia, Castillo Carlina, Guerra Martha
Grupo de Nutrición, Subdirección de Investigación, Instituto Nacional de Salud, Bogotá, DC, Colombia.
Biomedica. 2007 Dec;27(4):526-36.
Research in laboratories around the world has documented the contribution of the E apolipoprotein alleles to structural variations of lipids and apolipoproteins.
The gene frequencies of the E apolipoprotein alleles were compared with the lipid and apolipoprotein levels in school age children.
Six hundred and ninety one 5 to 15 years old school age children from the Colombian departments of Cundinamarca, Boyacá, Meta, Santander and Norte de Santander, were evaluated. The genotypes of the E apolipoprotein were identified by polymerase chain reaction-restriction fragment length polymorphism. Plasma levels for the following 5 lipids and lipoproteins were assayed: total cholesterol, HDL (high density lipoprotein) cholesterol, LDL (low density lipoprotein) cholesterol, triglycerides, VLDL (very low density lipoprotein) cholesterol, A-I apolipoprotein and B-100 apolipoprotein.
Alleles e2, e3 and e4 were found in frequencies of 0.04, 0.86 and 0.08, respectively. The E4 group (E4/3-E4/4), in contrast with the E2 group (E3/2-E2/2), presented highest plasma concentrations of total cholesterol, LDL cholesterol and B-100 apolipoprotein (p=0.014, 0.001 and 0.000, respectively). When the E3/3 group was compared with E2, the same result was obtained (p=0.015, 0.002 and 0.002, respectively). The influence of the E apolipoprotein polymorphism appeared greater in female children than male.
The e4 allele was associated with higher levels of total cholesterol, LDL cholesterol and B-100 apolipoprotein and indicates the necessity of additional research into the interactions between polymorphism E apolipoprotein and other genes, life styles, risk factors and potential contribution to cardiovascular diseases.
世界各地实验室的研究已证明载脂蛋白E等位基因对脂质和载脂蛋白结构变异的作用。
比较学龄儿童中载脂蛋白E等位基因的基因频率与脂质和载脂蛋白水平。
对来自哥伦比亚昆迪纳马卡省、博亚卡省、梅塔省、桑坦德省和北桑坦德省的691名5至15岁学龄儿童进行了评估。通过聚合酶链反应-限制性片段长度多态性鉴定载脂蛋白E的基因型。检测了以下5种脂质和脂蛋白的血浆水平：总胆固醇、高密度脂蛋白（HDL）胆固醇、低密度脂蛋白（LDL）胆固醇、甘油三酯、极低密度脂蛋白（VLDL）胆固醇、载脂蛋白A-I和载脂蛋白B-100。
发现等位基因e2、e3和e4的频率分别为0.04、0.86和0.08。与E2组（E3/2-E2/2）相比，E4组（E4/3-E4/4）的总胆固醇、LDL胆固醇和载脂蛋白B-100的血浆浓度最高（分别为p=0.014、0.001和0.000）。当将E3/3组与E2组进行比较时，得到了相同的结果（分别为p=0.015、0.002和0.002）。载脂蛋白E多态性对女童的影响似乎大于男童。
e4等位基因与较高水平的总胆固醇、LDL胆固醇和载脂蛋白B-100相关，这表明有必要进一步研究载脂蛋白E多态性与其他基因、生活方式、危险因素之间的相互作用以及对心血管疾病的潜在影响。
