突触前钙电流及其与突触传递的关系：乌贼巨大突触的电压钳研究及钙通道的理论模型
Presynaptic calcium currents and their relation to synaptic transmission: voltage clamp study in squid giant synapse and theoretical model for the calcium gate.
作者信息
Llinás R, Steinberg I Z, Walton K
出版信息
Proc Natl Acad Sci U S A. 1976 Aug;73(8):2918-22. doi: 10.1073/pnas.73.8.2918.
Abstract
A voltage clamp study of the presynaptic terminal in squid stellate ganglion has given quantitative results relating inward Ca2+ current to presynaptic membrane potential and postsynaptic response to inward Ca2+ current. The results indicate an S-shaped curve for the relationship between presynaptic potential and Ca2+ current and a linear relationship between Ca2+ current and postsynaptic potential. A similar S-shaped curve was found for the time-dependent properties of the Ca2+ conductance. Based on these results a mathematical model was developed which accounts for the experimental results in this and previously published papers by other authors. The model suggests that five subunits are involved in the Ca2+ gate and that the subunits change noncooperatively from an inactive to an active form upon membrane depolarization.
摘要
对鱿鱼星状神经节突触前终末进行的电压钳研究给出了定量结果，这些结果将内向Ca2+电流与突触前膜电位以及突触后反应与内向Ca2+电流联系起来。结果表明，突触前电位与Ca2+电流之间的关系呈S形曲线，而Ca2+电流与突触后电位之间呈线性关系。对于Ca2+电导的时间依赖性特性，也发现了类似的S形曲线。基于这些结果，建立了一个数学模型，该模型解释了本研究以及其他作者先前发表论文中的实验结果。该模型表明，Ca2+通道由五个亚基组成，膜去极化时，这些亚基以非协同方式从无活性形式转变为活性形式。
相似文献
1
Presynaptic calcium currents and their relation to synaptic transmission: voltage clamp study in squid giant synapse and theoretical model for the calcium gate.突触前钙电流及其与突触传递的关系：乌贼巨大突触的电压钳研究及钙通道的理论模型
Proc Natl Acad Sci U S A. 1976 Aug;73(8):2918-22. doi: 10.1073/pnas.73.8.2918.
2
Presynaptic calcium currents in squid giant synapse.鱿鱼巨大突触中的突触前钙电流。
Biophys J. 1981 Mar;33(3):289-321. doi: 10.1016/S0006-3495(81)84898-9.
3
Transmission in the squid giant synapse: a model based on voltage clamp studies.乌贼巨大突触中的传递：基于电压钳研究的模型。
J Physiol (Paris). 1980 Sep;76(5):413-8.
4
Transmission at voltage-clamped giant synapse of the squid: evidence for cooperativity of presynaptic calcium action.枪乌贼电压钳制巨突触的传递：突触前钙作用协同性的证据。
Proc Natl Acad Sci U S A. 1985 Jan;82(2):622-5. doi: 10.1073/pnas.82.2.622.
5
Role of presynaptic calcium ions and channels in synaptic facilitation and depression at the squid giant synapse.突触前钙离子及通道在枪乌贼巨大突触的突触易化和抑制中的作用。
J Physiol. 1982 Feb;323:173-93. doi: 10.1113/jphysiol.1982.sp014067.
6
Calcium entry and transmitter release at voltage-clamped nerve terminals of squid.枪乌贼电压钳制神经末梢处的钙内流与递质释放
J Physiol. 1985 Oct;367:163-81. doi: 10.1113/jphysiol.1985.sp015819.
7
Regulation by synapsin I and Ca(2+)-calmodulin-dependent protein kinase II of the transmitter release in squid giant synapse.突触结合蛋白I和钙调蛋白依赖性蛋白激酶II对枪乌贼巨大突触中递质释放的调节作用。
J Physiol. 1991 May;436:257-82. doi: 10.1113/jphysiol.1991.sp018549.
8
Presynaptic calcium channels and the depletion of synaptic cleft calcium ions.突触前钙通道与突触间隙钙离子耗竭。
J Neurophysiol. 2000 Jan;83(1):477-82. doi: 10.1152/jn.2000.83.1.477.
9
The voltage-dependence of transmitter release.递质释放的电压依赖性。
J Physiol (Paris). 1986;81(4):332-9.
10
Calcium role in depolarization-secretion coupling: an aequorin study in squid giant synapse.钙在去极化-分泌偶联中的作用：利用水母发光蛋白对鱿鱼巨大突触的研究
Proc Natl Acad Sci U S A. 1975 Jan;72(1):187-90. doi: 10.1073/pnas.72.1.187.
引用本文的文献
1
Pathological Interplay between Inflammation and Mitochondria Aggravates Glutamate Toxicity.炎症与线粒体的病理性相互作用加剧了谷氨酸毒性。
Int J Mol Sci. 2024 Feb 14;25(4):2276. doi: 10.3390/ijms25042276.
2
PLC regulates spontaneous glutamate release triggered by extracellular calcium and readily releasable pool size in neocortical neurons.磷脂酶C调节新皮质神经元中由细胞外钙触发的自发性谷氨酸释放以及易释放池的大小。
Front Cell Neurosci. 2023 May 16;17:1193485. doi: 10.3389/fncel.2023.1193485. eCollection 2023.
3
A negative feedback loop in the GPCR pathway underlies efficient coding of external stimuli.G 蛋白偶联受体通路中的负反馈环是外部刺激有效编码的基础。
Mol Syst Biol. 2022 Sep;18(9):e10514. doi: 10.15252/msb.202110514.
4
PrP as a Transducer of Physiological and Pathological Signals.朊蛋白作为生理和病理信号的转导分子。
Front Mol Neurosci. 2021 Nov 22;14:762918. doi: 10.3389/fnmol.2021.762918. eCollection 2021.
5
Functions of Presynaptic Voltage-gated Calcium Channels.突触前电压门控钙通道的功能。
Function (Oxf). 2021;2(1):zqaa027. doi: 10.1093/function/zqaa027. Epub 2020 Oct 23.
6
Targeted Delivery of Mitochondrial Calcium Channel Regulators: The Future of Glaucoma Treatment?线粒体钙通道调节剂的靶向递送：青光眼治疗的未来？
Front Neurosci. 2017 Nov 22;11:648. doi: 10.3389/fnins.2017.00648. eCollection 2017.
7
Synapse fits neuron: joint reduction by model inversion.突触拟合神经元：通过模型反演进行关节复位
Biol Cybern. 2017 Aug;111(3-4):309-334. doi: 10.1007/s00422-017-0722-1. Epub 2017 Jul 8.
8
Accumulation of K in the synaptic cleft modulates activity by influencing both vestibular hair cell and calyx afferent in the turtle.突触间隙中钾离子的积累通过影响海龟的前庭毛细胞和杯状传入神经来调节活动。
J Physiol. 2017 Feb 1;595(3):777-803. doi: 10.1113/JP273060. Epub 2016 Nov 4.
9
Glutamate Release.谷氨酸释放
Neurochem Res. 2015 Dec;40(12):2443-60. doi: 10.1007/s11064-015-1622-1. Epub 2015 May 27.
10
Estimation of presynaptic calcium currents and endogenous calcium buffers at the frog neuromuscular junction with two different calcium fluorescent dyes.使用两种不同的钙荧光染料对青蛙神经肌肉接头处的突触前钙电流和内源性钙缓冲剂进行估计。
Front Synaptic Neurosci. 2015 Jan 7;6:29. doi: 10.3389/fnsyn.2014.00029. eCollection 2014.
本文引用的文献
1
TETRODOTOXIN BLOCKAGE OF SODIUM CONDUCTANCE INCREASE IN LOBSTER GIANT AXONS.河豚毒素对龙虾巨轴突中钠电导增加的阻断作用
J Gen Physiol. 1964 May;47(5):965-74. doi: 10.1085/jgp.47.5.965.
2
Electrical changes in pre- and postsynaptic axons of the giant synapse of Loligo.枪乌贼巨大突触的突触前和突触后轴突中的电变化。
J Gen Physiol. 1962 Jul;45(6):1181-93. doi: 10.1085/jgp.45.6.1181.
3
Movements of labelled calcium in squid giant axons.标记钙在枪乌贼巨大轴突中的移动。
J Physiol. 1957 Sep 30;138(2):253-81. doi: 10.1113/jphysiol.1957.sp005850.
4
The end-plate potential in mammalian muscle.哺乳动物肌肉中的终板电位。
J Physiol. 1956 Apr 27;132(1):74-91. doi: 10.1113/jphysiol.1956.sp005503.
5
Quantal components of the end-plate potential.终板电位的量子成分。
J Physiol. 1954 Jun 28;124(3):560-73. doi: 10.1113/jphysiol.1954.sp005129.
6
A quantitative description of membrane current and its application to conduction and excitation in nerve.膜电流的定量描述及其在神经传导和兴奋中的应用。
J Physiol. 1952 Aug;117(4):500-44. doi: 10.1113/jphysiol.1952.sp004764.
7
Co-operative action a calcium ions in transmitter release at the neuromuscular junction.钙离子在神经肌肉接头处递质释放中的协同作用。
J Physiol. 1967 Nov;193(2):419-32. doi: 10.1113/jphysiol.1967.sp008367.
8
The action of calcium on neuronal synapses in the squid.钙对鱿鱼神经元突触的作用。
J Physiol. 1966 May;184(2):473-98. doi: 10.1113/jphysiol.1966.sp007927.
9
Depolarization and calcium entry in squid giant axons.枪乌贼巨大轴突中的去极化和钙内流。
J Physiol. 1971 Nov;218(3):709-55. doi: 10.1113/jphysiol.1971.sp009641.
10
Calcium inward currents in internally perfused giant axons.内部灌流的巨大轴突中的内向钙电流。
J Physiol. 1973 Nov;235(1):225-65. doi: 10.1113/jphysiol.1973.sp010386.
Zotero 插件