Grissom F E, Brooks C L, Littleton G K
Department of Physiology and Biophysics, Howard University College of Medicine, Washington, D.C. 20059.
Endocr Res. 1990;16(1):51-75. doi: 10.1080/07435809009035920.
This study examines the effect of placenta on the evolution of lactogen receptor in virgin pseudopregnant rabbit ovary, adrenal gland and mammary gland. Pseudopregnancy was induced with human chorionic gonadotropin. Does were injected with vehicle or placenta daily beginning on day six of the pseudopregnancy. Vehicle-treated rabbits during pseudopregnancy demonstrated a peak of ovarian lactogen receptor on day eight of pseudopregnancy. After treatment with placental homogenate a shift of this peak to twenty days of pseudopregnancy occurred. Lactogen receptor in adrenal and mammary gland membranes had peak receptor concentrations on day 14 of pseudopregnancy. Injection of placenta induced a shift to day 17 and days 17-20 in mammary and adrenal membranes, respectively. Serum concentrations of progesterone, estradiol, 20 alpha dihydroprogesterone and prolactin in placenta-treated groups were not significantly different from those of vehicle-treated groups. Treatment of pseudopregnant does with a composite of hormones at the concentrations found in placental homogenate produced no modulation of tissue lactogen receptor. Fractionation of 20-day pregnant rabbit placenta revealed that 80% of this activity could be found in the acetone extract while 20% was in the bicarbonate extract. These observations suggest that increases of lactogen receptor in ovary, adrenal and mammary glands occur during pseudopregnancy in rabbits and it is further concluded that placenta can alter these receptor induction patterns to ones similar to those seen in these tissues during pregnancy.
本研究考察了胎盘对未孕假孕兔卵巢、肾上腺和乳腺中催乳素受体演变的影响。用人绒毛膜促性腺激素诱导假孕。从假孕第6天开始，每天给母兔注射溶媒或胎盘。假孕期间接受溶媒处理的兔子在假孕第8天卵巢催乳素受体出现峰值。用胎盘匀浆处理后，该峰值转移至假孕第20天。肾上腺和乳腺膜中的催乳素受体在假孕第14天受体浓度达到峰值。注射胎盘分别使乳腺和肾上腺膜中的峰值转移至第17天和第17 - 20天。胎盘处理组的孕酮、雌二醇、20α - 二氢孕酮和催乳素的血清浓度与溶媒处理组无显著差异。用胎盘匀浆中发现的浓度的激素复合物处理假孕母兔，未对组织催乳素受体产生调节作用。对怀孕20天的兔胎盘进行分级分离显示，80%的这种活性可在丙酮提取物中发现，而20%在碳酸氢盐提取物中。这些观察结果表明，兔子假孕期间卵巢、肾上腺和乳腺中的催乳素受体增加，并且进一步得出结论，胎盘可将这些受体诱导模式改变为与怀孕时这些组织中所见模式相似的模式。
