Yoshida Makoto, Liu Yuan, Uchida Satoshi, Kawarada Kensuke, Ukagami Yusuke, Ichinose Hitomi, Kaneko Satoshi, Fukuda Kiyoharu
Faculty of Agriculture, Tokyo University of Agriculture and Technology, Fuchu, Tokyo 183-8509, Japan.
Biosci Biotechnol Biochem. 2008 Mar;72(3):805-10. doi: 10.1271/bbb.70689. Epub 2008 Mar 7.
The effects of cellulose crystallinity, hemicellulose, and lignin on the enzymatic hydrolysis of Miscanthus sinensis to monosaccharides were investigated. A air-dried biomass was ground by ball-milling, and the powder was separated into four fractions by passage through a series of sieves with mesh sizes 250-355 microm, 150-250 microm, 63-150 microm, and <63 microm. Each fraction was hydrolyzed with commercially available cellulase and beta-glucosidase. The yield of monosaccharides increased as the crystallinity of the substrate decreased. The addition of xylanase increased the yield of both pentoses and glucose. Delignification by the sodium chlorite method improved the initial rate of hydrolysis by cellulolytic enzymes significantly, resulting in a higher yield of monosaccharides as compared with that for untreated samples. When delignified M. sinensis was hydrolyzed with cellulase, beta-glucosidase, and xylanase, hemicellulose was hydrolyzed completely into monosaccharides, and the conversion rate of glucan to glucose was 90.6%.
研究了纤维素结晶度、半纤维素和木质素对芒草酶解生成单糖的影响。将风干的生物质通过球磨进行研磨，然后将粉末通过一系列孔径为250 - 355微米、150 - 250微米、63 - 150微米和<63微米的筛网分成四个级分。每个级分用市售的纤维素酶和β-葡萄糖苷酶进行水解。随着底物结晶度的降低，单糖产率增加。添加木聚糖酶提高了戊糖和葡萄糖的产率。用亚氯酸钠法进行脱木质素处理显著提高了纤维素分解酶的初始水解速率，与未处理样品相比，单糖产率更高。当用纤维素酶、β-葡萄糖苷酶和木聚糖酶对脱木质素的芒草进行水解时，半纤维素完全水解成单糖，葡聚糖转化为葡萄糖的转化率为90.6%。
