Institute of Microbiology, Beijing Forestry University, Beijing 100083, China.
Bioresour Technol. 2013 Apr;134:381-5. doi: 10.1016/j.biortech.2013.02.042. Epub 2013 Feb 21.
Selective delignification and hemicellulose removal were performed on white rot fungus-pretreated residues to investigate the effects of lignin and hemicellulose removal on enzymatic hydrolysis. 43.66-77% of lignin with small part of hemicellulose were degraded by chlorite treatment, while 79.97-95.09% of hemicellulose with little lignin were degraded by dilute acid treatment, indicating that cross effect between lignin and hemicellulose was minimized. In subsequent enzymatic digestion, regardless of the cellulase loading, residues from series-grade delignification released more glucose and xylose than that from hemicellulose removal, suggesting that lignin rather than hemicellulose in fungi-pretreated residues played a dominant role in hindering enzymatic hydrolysis. Based on the fundamental mechanisms of acidic/alkaline pretreatments in literature, it is proposed that fungal pretreatment prefers to integrate with alkaline pretreatment rather than acidic pretreatment to maximize the synergy. This indication would be helpful to optimize and renovate the integrated pretreatment.
采用选择性脱木质素和半纤维素的方法对白腐菌预处理后的残渣进行处理，以研究脱除木质素和半纤维素对酶解的影响。亚氯酸盐处理可降解 43.66-77%的木质素和少量的半纤维素，稀酸处理可降解 79.97-95.09%的半纤维素和少量的木质素，表明木质素和半纤维素之间的交叉效应最小化。在随后的酶解中，无论纤维素酶的加载量如何，系列级脱木质素后的残渣释放的葡萄糖和木糖都比半纤维素去除后的残渣多，这表明木质素而不是真菌预处理后的残渣中的半纤维素在阻碍酶解中起主导作用。根据文献中酸性/碱性预处理的基本机制，提出真菌预处理更倾向于与碱性预处理相结合，而不是与酸性预处理相结合，以最大限度地发挥协同作用。这一指示将有助于优化和改造集成预处理。
