Department of Forest Biomaterials, North Carolina State University, Raleigh, NC 27695-8005, USA.
Bioresour Technol. 2011 Oct;102(19):9083-9. doi: 10.1016/j.biortech.2011.07.001. Epub 2011 Jul 14.
The effect of delignification methods on enzymatic hydrolysis of forest biomass was investigated using softwood and hardwood that were pretreated at an alkaline condition followed by sodium chlorite or ozone delignification. Both delignifications improved enzymatic hydrolysis especially for softwood, while pretreatment alone was found effective for hardwood. High enzymatic conversion was achieved by sodium chlorite delignification when the lignin content was reduced to 15%, which is corresponding to 0.30-0.35 g/g accessible pore volume, and further delignification showed a marginal effect. Sample crystallinity index increased with lignin removal, but it did not show a correlation with the overall carbohydrate conversion of enzymatic hydrolysis.
研究了用碱性预处理后再用亚氯酸钠或臭氧脱木质素的方法对森林生物质进行酶水解的效果。两种脱木质素方法都显著提高了酶水解效率，特别是对软木，而单独预处理对硬木也很有效。当木质素含量降低到 15%时（相当于可及孔体积的 0.30-0.35 g/g），通过亚氯酸钠脱木质素可实现高酶转化，进一步脱木质素的效果则微不足道。样品结晶度指数随木质素去除而增加，但与酶水解的总碳水化合物转化率没有相关性。
