Yukphan Pattaraporn, Malimas Taweesak, Muramatsu Yuki, Takahashi Mai, Kaneyasu Mika, Tanasupawat Somboon, Nakagawa Yasuyoshi, Suzuki Ken-ichiro, Potacharoen Wanchern, Yamada Yuzo
BIOTEC Culture Collection, National Center for Genetic Engineering and Biotechnology (BIOTEC), Pathumthani 12120, Thailand.
Biosci Biotechnol Biochem. 2008 Mar;72(3):672-6. doi: 10.1271/bbb.70319. Epub 2008 Mar 7.
Tanticharoenia sakaeratensis gen. nov., sp. nov. is proposed for three strains isolated from soil collected in Thailand. The three strains, AC37(T), AC38, and AC39, were included within a lineage comprising the genera Asaia, Kozakia, Swaminathania, Neoasaia, Acetobacter, Gluconobacter, and Saccharibacter in a phylogenetic tree based on 16S rRNA gene sequences, but formed a quite different, independent cluster. Pair-wise sequence similarities of strain AC37(T) were 96.5-92.1% to the type strains of Acetobacter aceti, Gluconobacter oxydans, Acidomonas methanolica, Gluconacetobacter liquefaciens, Asaia bogorensis, Kozakia baliensis, Swaminathania salitolerans, Saccharibacter floricola, Neoasaia chiangmaiensis, and Granulibacter bethesdensis. The three strains had DNA base compositions comprising respectively 65.6, 64.5, and 65.6 mol % G+C with a range of 1.1 mol %, and formed a single species. Phenotypically, the three strains did not oxidize acetate or lactate, but grew on 30% D-glucose (w/v). Chemotaxonomically, they had Q-10. The type strain is AC37(T) (= BCC 15772(T) = NBRC 103193(T)).
为从泰国采集的土壤中分离出的三株菌株提议建立新属——萨凯拉特坦蒂恰罗尼亚菌属（Tanticharoenia）及新种——萨凯拉特坦蒂恰罗尼亚菌（Tanticharoenia sakaeratensis）。这三株菌株，即AC37(T)、AC38和AC39，在基于16S rRNA基因序列构建的系统发育树中，位于包含醋杆菌属（Asaia）、科扎基亚菌属（Kozakia）、斯瓦米纳坦尼亚菌属（Swaminathania）、新醋杆菌属（Neoasaia）、醋酸杆菌属（Acetobacter）、葡糖杆菌属（Gluconobacter）和糖杆菌属（Saccharibacter）的一个分支内，但形成了一个截然不同的独立簇。菌株AC37(T)与醋化醋杆菌（Acetobacter aceti）、氧化葡糖杆菌（Gluconobacter oxydans）、甲醇嗜酸菌（Acidomonas methanolica）、液化葡糖醋杆菌（Gluconacetobacter liquefaciens）、博戈里亚醋杆菌（Asaia bogorensis）、巴厘岛科扎基亚菌（Kozakia baliensis）、耐盐斯瓦米纳坦尼亚菌（Swaminathania salitolerans）、栖花糖杆菌（Saccharibacter floricola）、清迈新醋杆菌（Neoasaia chiangmaiensis）和贝塞斯达颗粒杆菌（Granulibacter bethesdensis）的模式菌株的成对序列相似性为96.5 - 92.1%。这三株菌株的DNA碱基组成为G+C分别为65.6、64.5和65.6 mol%，范围为1.1 mol%，并形成一个单一物种。从表型上看，这三株菌株不氧化醋酸盐或乳酸盐，但能在30% D - 葡萄糖（w/v）上生长。从化学分类学上看，它们含有泛醌Q - 10。模式菌株为AC37(T)（= BCC 15772(T) = NBRC 103193(T)）。
