LeMaire W J, Davies P J, Marsh J M
Prostaglandins. 1976 Aug;12(2):271-9. doi: 10.1016/0090-6980(76)90121-0.
The possibility that prostaglandins might be responsible for the development of the pre-ovulatory refractoriness to the stimulation by LH of cyclic AMP accumulation in vitro in the Graafian follicle was examined. Isolated rabbit Graafian follicles were obtained at estrus and at 0.5, 5 and 9 hours after an ovulatory dose of LH. The follicles were incubated in vitro in the presence of (8-3H)adenine and the accumulation of (8-3H)cyclic AMP measured. Follicles from estrous animals responded to the in vitro addition of LH with a marked increase of cyclic AMP accumulation and lost this response as the time of ovulation approached. Animals pretreated with indomethacin, which inhibits the usual pre-ovulatory rise of follicular prostaglandin levels, showed essentially the same loss of responsiveness. Indomethacin alone was without effect. It is concluded that prostaglandins are not the major factor in the development of refractoriness to LH stimulation in vitro which has been observed in pre-ovulatory follicles.
研究了前列腺素是否可能是导致排卵前卵泡对促黄体生成素（LH）刺激体外积累环磷酸腺苷（cAMP）产生不应性的原因。在发情期以及给予排卵剂量LH后的0.5、5和9小时获取分离的兔格拉夫卵泡。将卵泡在（8-³H）腺嘌呤存在的情况下进行体外培养，并测定（8-³H）cAMP的积累。来自发情动物的卵泡对体外添加的LH有反应，cAMP积累显著增加，并且随着排卵时间临近，这种反应消失。用吲哚美辛预处理的动物，吲哚美辛可抑制卵泡前列腺素水平通常的排卵前升高，其显示出基本相同的反应性丧失。单独使用吲哚美辛没有效果。得出的结论是，前列腺素不是在排卵前卵泡中观察到的对LH刺激体外不应性发展的主要因素。
