Mingorance Carmen, Andriantsitohaina Ramaroson, Alvarez de Sotomayor Maria
Department of Pharmacology, Faculty of Pharmacy, University of Seville, Spain.
J Ethnopharmacol. 2008 Apr 17;117(1):76-83. doi: 10.1016/j.jep.2008.01.019. Epub 2008 Feb 2.
Cedrelopsis grevei Baill. (Ptaeroxylaceae) trunk bark extract is empirically used in Madagascar against several pathologies, from persistent catarrh to hypertension. The effect C. grevei extract on age-related changes in systolic blood pressure (SBP) and endothelial function was investigated. Rats (90-100 week-old) received treatment either with C. grevei extract (80 mg kg(-1)) or vehicle for 8 weeks. SBP was evaluated by tail-cuff and vascular reactivity and endothelial vasodilatation of both aortae and small mesenteric arteries (SMA) were assessed by acetylcholine (ACh) in the presence or in the absence of either reactive oxygen species (ROS) scavengers superoxide dismutase (SOD) plus catalase or the nitric oxide synthase inhibitor, NG-L-arginine methyl ester (L-NAME). Plasma nitric oxide (NO) was evaluated by nitrite assay and expressions of eNOS, Cu/Zn-, Mn- and EC-SOD were determined by Western Blot. C. grevei administration prevented the increase of SBP and improved endothelium-dependent relaxations in aortae and SMA from aged rat via increased NO and decreased participation of ROS. Furthermore, C. grevei treatment enhanced plasma nitrite content but did not modify eNOS, Cu/Zn-, Mn- or EC-SOD expressions in the two arteries studied. These results suggest that C. grevei prevents both increased blood pressure and age-related endothelial dysfunction supporting the empirical use of C. grevei trunk bark extract against mild hypertension often associated with aging.
贝利氏木（Cedrelopsis grevei Baill.，苦木科）树干树皮提取物在马达加斯加被经验性地用于治疗多种病症，从持续性粘膜炎到高血压。研究了贝利氏木提取物对收缩压（SBP）与年龄相关变化以及内皮功能的影响。给90 - 100周龄的大鼠分别用贝利氏木提取物（80 mg·kg⁻¹）或赋形剂处理8周。通过尾套法评估SBP，在有或无活性氧（ROS）清除剂超氧化物歧化酶（SOD）加过氧化氢酶或一氧化氮合酶抑制剂N⁻L⁻精氨酸甲酯（L⁻NAME）的情况下，用乙酰胆碱（ACh）评估主动脉和小肠系膜小动脉（SMA）的血管反应性和内皮血管舒张功能。通过亚硝酸盐测定评估血浆一氧化氮（NO），通过蛋白质免疫印迹法测定内皮型一氧化氮合酶（eNOS）、铜/锌超氧化物歧化酶、锰超氧化物歧化酶和细胞外超氧化物歧化酶（EC - SOD）的表达。给予贝利氏木提取物可预防老年大鼠主动脉和SMA中SBP的升高，并通过增加NO和减少ROS的参与改善内皮依赖性舒张。此外，贝利氏木提取物处理可提高血浆亚硝酸盐含量，但不改变所研究的两条动脉中eNOS、铜/锌超氧化物歧化酶、锰超氧化物歧化酶或EC - SOD的表达。这些结果表明，贝利氏木可预防血压升高和与年龄相关的内皮功能障碍，支持将贝利氏木树干树皮提取物经验性地用于治疗常与衰老相关的轻度高血压。
