Campana Priscilla R V, Braga Fernão C, Cortes Steyner F
Faculdade de Farmácia, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
Phytomedicine. 2009 May;16(5):456-61. doi: 10.1016/j.phymed.2008.09.007. Epub 2008 Nov 18.
The vasodilator effect of the ethanolic extract of Mansoa hirsuta leaves (EEF) was assayed in rat aortic rings. EEF produced a concentration-dependent vasodilatation (pIC(50)=5.1+/-0.2), which was absent in endothelium-denuded vessels. The vasodilator effect of EEF was similar to a standardized ethanolic extract of Hancornia speciosa Gomes (pIC(50)=5.1+/-0.1). The endothelium-dependent vasodilatation induced by EEF was abolished by L-NAME (100 microM), a nitric oxide (NO) synthase inhibitor, but not by indomethacin (10 microM; pIC(50)=4.9+/-0.2), a cyclooxygenase inhibitor. The concentration-response curve of EEF was not modified by the addition of superoxide dismutase (SOD; 300 U/ml). In addition, EEF (50 microg/ml) displaced the 3-morpholino-sidnonimine (SIN-1; p<0.05) concentration-effect curve to the left, as well as SOD (300 U/ml). These findings lead us to conclude that EEF induces a NO- and endothelium-dependent vasodilatation in rat aortic preparations, and that this effect is, at least in some extent, due to an increase in the NO bioavailability as consequence of its antioxidant activity. The HPLC-DAD profile recorded for EEF indicates the presence of four major peaks with close retention times, exhibiting similar UV spectra with wavelength maxima compatible with heterogeneous proanthocyanidins.
对曼索阿多毛叶乙醇提取物（EEF）的血管舒张作用在大鼠主动脉环中进行了测定。EEF产生浓度依赖性血管舒张作用（pIC(50)=5.1±0.2），而在内皮剥脱的血管中则不存在这种作用。EEF的血管舒张作用与汉考尼亚特殊戈麦斯的标准化乙醇提取物相似（pIC(50)=5.1±0.1）。EEF诱导的内皮依赖性血管舒张作用被一氧化氮（NO）合酶抑制剂L-NAME（100 microM）消除，但不被环氧化酶抑制剂吲哚美辛（10 microM；pIC(50)=4.9±0.2）消除。添加超氧化物歧化酶（SOD；300 U/ml）后，EEF的浓度-反应曲线未发生改变。此外，EEF（50 microg/ml）使3-吗啉代-西多非尼（SIN-1；p<0.05）的浓度-效应曲线向左移动，SOD（300 U/ml）也有同样效果。这些发现使我们得出结论，EEF在大鼠主动脉制剂中诱导了一种NO和内皮依赖性血管舒张作用，并且这种作用至少在一定程度上是由于其抗氧化活性导致NO生物利用度增加所致。记录的EEF的HPLC-DAD图谱表明存在四个保留时间相近的主要峰，其紫外光谱相似，波长最大值与异源原花青素相符。
