Heinisch J, Vogelsang K, Hollenberg C P
Institut für Mikrobiologie, Heinrich-Heine-Universität Düsseldorf, Germany.
FEBS Lett. 1991 Sep 2;289(1):77-82. doi: 10.1016/0014-5793(91)80912-m.
We here provide the complete nucleotide sequences of the 5'-non-coding regions of the yeast phosphofructokinase genes, PFK1 and PFK2. lacZ fusions of the PFK1 and PFK2 promoters were constructed and a deletion analysis was performed. In contrast to other glycolytic gene promoters, no strong regulatory elements could be found. However, we detected moderate UAS and URS functions. In general, the effects on expression upon deletion of these regions were more pronounced on media containing ethanol than on those containing glucose as carbon sources. Overexpression of either one of the PFK genes led to a decreased enzymatic activity in a wild-type background but did not affect transcription from the promoters.
我们在此提供酵母磷酸果糖激酶基因PFK1和PFK2的5'-非编码区的完整核苷酸序列。构建了PFK1和PFK2启动子的lacZ融合体并进行了缺失分析。与其他糖酵解基因启动子不同，未发现强调控元件。然而，我们检测到了适度的上游激活序列（UAS）和上游阻遏序列（URS）功能。一般来说，在以乙醇为碳源的培养基上，缺失这些区域对表达的影响比在以葡萄糖为碳源的培养基上更明显。在野生型背景下，任一PFK基因的过表达都会导致酶活性降低，但不影响启动子的转录。
