McKay Terri L, Gedeon Dan J, Vickerman Mary B, Hylton Alan G, Ribita Daniela, Olar Harry H, Kaiser Peter K, Parsons-Wingerter Patricia
Research & Technology Directorate and National Center for Space Exploration Research, NASA Glenn Research Center, Cleveland, Ohio 44135, USA.
Invest Ophthalmol Vis Sci. 2008 Mar;49(3):1184-90. doi: 10.1167/iovs.07-1329.
To quantify the effects of the steroid triamcinolone acetonide (TA) on branching morphology within the angiogenic microvascular tree of the chorioallantoic membrane (CAM) of quail embryos.
Increasing concentrations of TA (0-16 ng/mL) were applied topically on embryonic day (E) 7 to the chorioallantoic membrane (CAM) of quail embryos cultured in petri dishes and incubated for an additional 24 or 48 hours until fixation. Binary (black/white) microscopic images of arterial end points were quantified by generational analysis of vessel branching (VESGEN) software to obtain major vascular parameters that include vessel diameter (D(v)), fractal dimension (D(f)), tortuosity (T(v)), and densities of vessel area, length, number, and branch point (A(v), L(v), N(v), and Br(v)). For assessment of specific changes in vascular morphology induced by TA, the VESGEN software automatically segmented the vascular tree into branching generations (G(1)... G(10)) according to changes in vessel diameter and branching.
Vessel density decreased significantly up to 34% as the function of increasing concentration of TA according to A(v), L(v), Br(v), N(v), and D(f). TA selectively inhibited the growth of new, small vessels because L(v) decreased from 13.14 +/- 0.61 cm/cm(2) for controls to 8.012 +/- 0.82 cm/cm(2) at 16 ng TA/mL in smaller branching generations (G(7)-G(10)) and for N(v) from 473.83 +/- 29.85 cm(-2) to 302.32 +/- 33.09 cm(-2). In contrast, vessel diameter (D(v)) decreased throughout the vascular tree (G(1)-G(10)).
By VESGEN analysis, TA selectively inhibited the angiogenesis of smaller blood vessels, but decreased the vessel diameter of all vessels within the vascular tree.
量化类固醇曲安奈德（TA）对鹌鹑胚胎绒毛尿囊膜（CAM）血管生成微血管树分支形态的影响。
在胚胎第7天（E7），将浓度递增的TA（0 - 16 ng/mL）局部应用于培养在培养皿中的鹌鹑胚胎的绒毛尿囊膜（CAM），并再孵育24或48小时直至固定。通过血管分支（VESGEN）软件对动脉端点的二元（黑白）显微镜图像进行世代分析，以获得主要血管参数，包括血管直径（D(v)）、分形维数（D(f)）、曲折度（T(v)）以及血管面积、长度、数量和分支点的密度（A(v)、L(v)、N(v)和Br(v)）。为评估TA诱导的血管形态的特定变化，VESGEN软件根据血管直径和分支的变化自动将血管树分割为分支世代（G(1)... G(10)）。
根据A(v)、L(v)、Br(v)、N(v)和D(f)，随着TA浓度增加，血管密度显著降低达34%。TA选择性抑制新生小血管的生长，因为在较小分支世代（G(7) - G(10)）中，L(v)从对照组的13.14 ± 0.61 cm/cm²降至16 ng TA/mL时的8.012 ± 0.82 cm/cm²，N(v)从473.83 ± 29.85 cm⁻²降至302.32 ± 33.09 cm⁻²。相反，整个血管树（G(1) - G(10)）的血管直径（D(v)）均减小。
通过VESGEN分析，TA选择性抑制较小血管的血管生成，但降低了血管树内所有血管的直径。
