Kitazawa H, Nomura M, Itoh T, Yamaguchi T
Laboratory of Animal Products Chemistry, Laboratory of Animal Morphology, Faculty of Agriculture, Tohoku University, Sendai, Japan.
J Dairy Sci. 1991 Jul;74(7):2082-8. doi: 10.3168/jds.S0022-0302(91)78380-X.
The effect of a slime-forming, encapsulated Lactococcus lactis ssp. cremoris KVS20 on macrophage function has been examined in vivo and in vitro in short-term studies. Peritoneal macrophages in which 21 to 34% of macrophage was presenting Fc gamma-receptor positive macrophages were elicited by intraperitoneal injection of 10 to 50 mg/kg of L. lactis ssp. cremoris KVS20. The peritoneal macrophage exhibited cytotoxic activity against Sarcoma-180 cells in which the maximum activity was obtained in macrophage from mice injected with 10 mg/kg on d 5. However, L. lactis ssp. cremoris KVS20 rendered the elicited macrophage cytotoxic in vitro. The cytotoxicity was significantly augmented by 6- and 24-h treatment at the concentration of 50 to 500 micrograms/ml. These results obtained in the short-term studies demonstrated that the antitumor activity of L. lactis ssp. cremoris KVS20 may be mediated through the enhanced cytotoxic activity of macrophage.
在短期研究中，已在体内和体外检测了产黏液、有荚膜的乳酸乳球菌亚种乳脂乳球菌KVS20对巨噬细胞功能的影响。通过腹腔注射10至50mg/kg的乳脂乳球菌亚种KVS20，诱导出腹腔巨噬细胞，其中21%至34%的巨噬细胞呈现Fcγ受体阳性巨噬细胞。腹腔巨噬细胞对肉瘤-180细胞表现出细胞毒性活性，在第5天注射10mg/kg的小鼠巨噬细胞中获得最大活性。然而，乳脂乳球菌亚种KVS20使诱导的巨噬细胞在体外具有细胞毒性。在50至500μg/ml的浓度下，经过6小时和24小时处理后，细胞毒性显著增强。这些在短期研究中获得的结果表明，乳脂乳球菌亚种KVS20的抗肿瘤活性可能是通过增强巨噬细胞的细胞毒性活性来介导的。
