Kitazawa H, Yamaguchi T, Itoh T
Department of Animal Science, Faculty of Agriculture, Tohoku University, Sendai, Japan.
J Dairy Sci. 1992 Nov;75(11):2946-51. doi: 10.3168/jds.S0022-0302(92)78057-6.
The mitogenic activities of whole cell lyophylized preparations, cell-wall components, and slime products obtained from Lactococcus lactis ssp. cremoris KVS20 were examined on murine spleen cells. Whole cell lyophylized preparations and slime products significantly (P < .05) stimulated mitogenic responses of the cells. The highest activity was induced by slime products in which the optimal concentration was 116 microg/ml. The significant (P < .05) increase of mitogenic activity induced by slime products occurred at 24 h, and the peak response was obtained 48 h after the stimulation. The activity was much higher in the fraction enriched with B cells than in the fraction enriched with T cells. In addition, slime products induced mitogenic activity to spleen cells of athymic nu/nu mice. The chemical analysis of lipopolysaccharide and the minimal concentration for mitogenic response eliminated the possibility that the activity of slime products may be due to the contamination of lipopolysaccharide. The data demonstrate that slime products are a potent B-cell-dependent mitogen.
对从乳酸乳球菌乳脂亚种KVS20获得的全细胞冻干制剂、细胞壁成分和黏液产物在小鼠脾细胞上的促有丝分裂活性进行了检测。全细胞冻干制剂和黏液产物显著（P < 0.05）刺激了细胞的有丝分裂反应。黏液产物诱导的活性最高，其最佳浓度为116微克/毫升。黏液产物诱导的有丝分裂活性在24小时时显著（P < 0.05）增加，刺激后48小时获得峰值反应。富含B细胞的组分中的活性比富含T细胞的组分中的活性高得多。此外，黏液产物对无胸腺裸鼠的脾细胞诱导了有丝分裂活性。脂多糖的化学分析以及有丝分裂反应的最低浓度排除了黏液产物的活性可能归因于脂多糖污染的可能性。数据表明黏液产物是一种有效的B细胞依赖性有丝分裂原。
