Chen Doris, Ahlford Annika, Schnorrer Frank, Kalchhauser Irene, Fellner Michaela, Viràgh Erika, Kiss Istvàn, Syvänen Ann-Christine, Dickson Barry J
Research Institute of Molecular Pathology (IMP), Dr. Bohr-Gasse 7, A-1030 Vienna, Austria.
Nat Methods. 2008 Apr;5(4):323-9. doi: 10.1038/nmeth.1191. Epub 2008 Mar 9.
Single nucleotide polymorphisms (SNPs) are useful markers for genetic mapping experiments in model organisms. Here we report the establishment of a high-density SNP map and high-throughput genotyping assays for Drosophila melanogaster. Our map comprises 27,367 SNPs in common laboratory Drosophila stocks. These SNPs were clustered within 2,238 amplifiable markers at an average density of 1 marker every 50.3 kb, or 6.3 genes. We have also constructed a set of 62 Drosophila stocks, each of which facilitates the generation of recombinants within a defined genetic interval of 1-2 Mb. For flexible, high-throughput SNP genotyping, we used fluorescent tag-array mini-sequencing (TAMS) assays. We designed and validated TAMS assays for 293 SNPs at an average resolution of 391.3 kb, and demonstrated the utility of these tools by rapidly mapping 14 mutations that disrupt embryonic muscle patterning. These resources enable high-resolution high-throughput genetic mapping in Drosophila.
单核苷酸多态性（SNPs）是模式生物遗传图谱实验中有用的标记。在此，我们报告了黑腹果蝇高密度SNP图谱的建立和高通量基因分型分析方法。我们的图谱包含常见实验室果蝇品系中的27367个SNPs。这些SNPs聚集在2238个可扩增标记内，平均密度为每50.3 kb有1个标记，或每6.3个基因有1个标记。我们还构建了一组62个果蝇品系，每个品系都有助于在1 - 2 Mb的特定遗传区间内产生重组体。为了进行灵活的高通量SNP基因分型，我们使用了荧光标记阵列微测序（TAMS）分析方法。我们设计并验证了平均分辨率为391.3 kb的针对293个SNPs的TAMS分析方法，并通过快速定位14个破坏胚胎肌肉模式的突变证明了这些工具的实用性。这些资源能够在果蝇中进行高分辨率的高通量遗传图谱绘制。
