5'-氨基-5'-脱氧鸟苷-5'-N-磷酰胺的制备、纯化及其与T7 RNA聚合酶的起始特性
Preparation and purification of 5'-amino-5'-deoxyguanosine-5'-N-phosphoramidate and its initiation properties with T7 RNA polymerase.
作者信息
Williamson David, Hodgson David R W
机构信息
Department of Chemistry, University Science Laboratories, South Road, Durham, DH1 3LE, England.
出版信息
Org Biomol Chem. 2008 Mar 21;6(6):1056-62. doi: 10.1039/b717896d. Epub 2008 Feb 12.
5'-Amino-5'-deoxyguanosine-5'-N-phosphoramidate (GNHP) was synthesised in four steps from guanosine. Its identity was confirmed using spectroscopic and kinetic studies. A chromatographic method for the purification of this unstable compound was developed. GNHP was found to initiate T7 RNAP promoted transcriptions to afford 5'-O3P-NH-RNA, which hydrolyses readily to yield 5'-H2N-RNA that can be conjugated to activated esters.
5'-氨基-5'-脱氧鸟苷-5'-N-磷酰胺酸酯（GNHP）由鸟苷经四步合成。通过光谱和动力学研究确认了其结构。开发了一种用于纯化这种不稳定化合物的色谱方法。发现GNHP可引发T7 RNA聚合酶促进的转录反应，生成5'-O3P-NH-RNA，该产物易于水解生成5'-H2N-RNA，后者可与活化酯共轭。
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