5'-脱氧-5'-肼基鸟苷作为T7 RNA聚合酶催化转录的引发剂用于制备可标记RNA。
5'-deoxy-5'-hydrazinylguanosine as an initiator of T7 Rna polymerase-catalyzed transcriptions for the preparation of labeling-ready RNAs.
作者信息
Skipsey Mark, Hack Gordon, Hooper Thomas A, Shankey Mark C, Conway Louis P, Schröder Martin, Hodgson David R W
机构信息
a Department of Chemistry and Biophysical Sciences Institute , Durham University , Durham , United Kingdom.
出版信息
Nucleosides Nucleotides Nucleic Acids. 2013;32(12):670-81. doi: 10.1080/15257770.2013.851393.
Abstract
5'-deoxy-5'-hydrazinylguanosine was incorporated into the 5'-termini of RNA transcripts using T7 RNA polymerase. Transcriptions provided 5'-hydrazinyl-RNA that was readily labeled and purified. The use of fluorophore-labeled material was validated in an endoribonuclease activity assay.
摘要
使用T7 RNA聚合酶将5'-脱氧-5'-肼基鸟苷掺入RNA转录本的5'-末端。转录产生了易于标记和纯化的5'-肼基-RNA。在核糖核酸内切酶活性测定中验证了荧光团标记材料的使用。
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