Gyoeva F K, Gelfand V I
Institute of Protein Research, Academy of Sciences of the USSR, Pushchino, Moscow.
Nature. 1991 Oct 3;353(6343):445-8. doi: 10.1038/353445a0.
Intermediate filaments in most types of cultured cells coalign with microtubules. Depolymerization of microtubules results in collapse of vimentin and desmin intermediate filaments to the nucleus where they form a perinuclear cap. Collapse can also be induced by microinjection of antibodies against intermediate filament or microtubule proteins. Thus, two filament systems interact with each other. But the molecules mediating this interaction are unknown. One of the candidates for this role is a microtubule motor kinesin. Recent data showed that kinesin is involved in the plus end-directed movement of the membranous organelles along microtubules such as radial extension of lysosomes in macrophages and centrifugal movement of pigment in melanophores. Here we report that injection of the anti-kinesin antibody into human fibroblasts results in the redistribution of intermediate filaments to a tight perinuclear aggregate but had no effect on the distribution of microtubules. Thus, kinesin is involved not only in organelle movement but also in interaction of the two major cytoskeletal systems, intermediate filaments and microtubules.
在大多数类型的培养细胞中，中间丝与微管平行排列。微管解聚导致波形蛋白和结蛋白中间丝向细胞核塌陷，在细胞核处形成核周帽。针对中间丝或微管蛋白的抗体显微注射也可诱导塌陷。因此，两种丝系统相互作用。但介导这种相互作用的分子尚不清楚。该作用的候选分子之一是微管马达驱动蛋白。最近的数据表明，驱动蛋白参与膜性细胞器沿微管的正向运动，如巨噬细胞中溶酶体的径向延伸和黑素细胞中色素的离心运动。在此我们报道，将抗驱动蛋白抗体注射到人成纤维细胞中会导致中间丝重新分布形成紧密的核周聚集体，但对微管的分布没有影响。因此，驱动蛋白不仅参与细胞器运动，还参与两种主要细胞骨架系统，即中间丝和微管的相互作用。
