Loo Tip W, Clarke David M
Department of Medicine, University of Toronto, 1 King's College Circle, Rm. 7342, Medical Sciences Building, Toronto, Ont., Canada.
Arch Biochem Biophys. 2008 Aug 1;476(1):51-64. doi: 10.1016/j.abb.2008.02.025. Epub 2008 Mar 5.
The 49 human members of the ATP-binding cassette (ABC) family of proteins are involved in a wide range of activities such as active transport of compounds across membranes, extraction of compounds out of membranes, functioning as ion channels, or regulators of channel activity. Mutations and/or overexpression of many of the proteins can have adverse effects on health. A goal in the study of ABC proteins is to understand their mechanisms of action. This review will focus on the mutational approaches that have been used to study the structure and mechanisms of some ABC proteins.
ATP结合盒（ABC）蛋白家族的49个成员参与了广泛的活动，如化合物跨膜的主动运输、从膜中提取化合物、作为离子通道发挥作用或调节通道活性。许多这类蛋白质的突变和/或过表达会对健康产生不利影响。研究ABC蛋白的一个目标是了解它们的作用机制。本综述将聚焦于用于研究某些ABC蛋白结构和机制的突变方法。
