Li Jun-Zhu, Liu Hai-Yang, Lin Yi-Ju, Hao Xiao-Jiang, Ni Wei, Chen Chang-Xiang
State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, No. 132, Lanhei Road, Yunnan, Kunming 650204, PR China.
Steroids. 2008 Jul;73(6):594-600. doi: 10.1016/j.steroids.2008.01.015. Epub 2008 Jan 20.
Six new C(21) steroidal glycosides, named curassavosides A-F (3-8), were obtained from the aerial parts of Asclepias curassavica (Asclepiadaceae), along with two known oxypregnanes, 12-O-benzoyldeacylmetaplexigenin (1) and 12-O-benzoylsarcostin (2). By spectroscopic methods, the structures of the six new compounds were determined as 12-O-benzoyldeacylmetaplexigenin 3-O-beta-D-oleandropyranosyl-(1-->4)-beta-D-digitoxopyranoside (3), 12-O-benzoylsarcostin 3-O-beta-D-oleandropyranosyl-(1-->4)-beta-D-digitoxopyranoside (4), sarcostin 3-O-beta-D-oleandropyranosyl-(1-->4)-beta-D-canaropyranosyl-(1-->4)-beta-D-oleandropyranosyl-(1-->4)-beta-D-digitoxopyranoside (5), sarcostin 3-O-beta-D-oleandropyranosyl-(1-->4)-beta-D-canaropyranosyl-(1-->4)-beta-D-canaropyranosyl-(1-->4)-beta-D-digitoxopyranoside (6), 12-O-benzoyldeacylmetaplexigenin 3-O-beta-D-glucopyranosyl-(1-->4)-beta-D-oleandropyranosyl-(1-->4)-beta-D-canaropyranosyl-(1-->4)-beta-d-oleandropyranosyl-(1-->4)-beta-D-digitoxopyranoside (7), and 12-O-benzoylsarcostin 3-O-beta-D-glucopyranosyl-(1-->4)-beta-D-oleandropyranosyl-(1-->4)-beta-d-canaropyranosyl-(1-->4)-beta-D-oleandropyranosyl-(1-->4)-beta-D-digitoxopyranoside (8), respectively. All compounds (1-8) were tested for in vitro cytotoxicity; only compound 3 showed weak inhibitory activity against Raji and AGZY cell lines.
从马利筋（萝藦科）地上部分分离得到6个新的C(21)甾体苷，命名为库拉索苷A - F（3 - 8），同时还得到两个已知的氧化孕甾烷，12 - O - 苯甲酰去酰基美络孕烯醇酮（1）和12 - O - 苯甲酰肉珊瑚苷元（2）。通过光谱方法，确定这6个新化合物的结构分别为12 - O - 苯甲酰去酰基美络孕烯醇酮3 - O - β - D - 毛地黄毒糖基 - (1→4) - β - D - 洋地黄毒糖吡喃糖苷（3）、12 - O - 苯甲酰肉珊瑚苷元3 - O - β - D - 毛地黄毒糖基 - (1→4) - β - D - 洋地黄毒糖吡喃糖苷（4）、肉珊瑚苷元3 - O - β - D - 毛地黄毒糖基 - (1→4) - β - D - 卡那糖基 - (1→4) - β - D - 毛地黄毒糖基 - (1→4) - β - D - 洋地黄毒糖吡喃糖苷（5）、肉珊瑚苷元3 - O - β - D - 毛地黄毒糖基 - (1→4) - β - D - 卡那糖基 - (1→4) - β - D - 卡那糖基 - (1→4) - β - D - 洋地黄毒糖吡喃糖苷（6）、12 - O - 苯甲酰去酰基美络孕烯醇酮3 - O - β - D - 葡萄糖基 - (1→4) - β - D - 毛地黄毒糖基 - (1→4) - β - D - 卡那糖基 - (1→4) - β - D - 毛地黄毒糖基 - (1→4) - β - D - 洋地黄毒糖吡喃糖苷（7）和12 - O - 苯甲酰肉珊瑚苷元3 - O - β - D - 葡萄糖基 - (1→4) - β - D - 毛地黄毒糖基 - (1→4) - β - D - 卡那糖基 - (1→4) - β - D - 毛地黄毒糖基 - (1→4) - β - D - 洋地黄毒糖吡喃糖苷（8）。对所有化合物（1 - 8）进行了体外细胞毒性测试；只有化合物3对Raji和AGZY细胞系表现出微弱的抑制活性。
