Eberhardt Mirjam, Hoffmann Tal, Sauer Susanne K, Messlinger Karl, Reeh Peter W, Fischer Michael J M
Institute of Physiology and Pathophysiology, University of Erlangen-Nuernberg, Erlangen, Germany.
Neuropeptides. 2008 Jun;42(3):311-7. doi: 10.1016/j.npep.2008.01.002. Epub 2008 Mar 6.
Neuropeptides like calcitonin gene-related peptide (CGRP) and substance P are found in significant proportions of primary afferent neurons. Release of these neuropeptides as well as prostaglandin E(2) is an approved index for the activation of these primary afferents. Previous studies have used cultures of enzyme-treated and mechanically dissociated primary afferent neurons, fresh tissue slices or cubes. In the present study we demonstrate CGRP and prostaglandin E(2) release from intact isolated dorsal root and trigeminal ganglia. Stimulation with noxious heat, low pH, inflammatory mediators and high potassium concentration increased CGRP release. In conclusion, neuropeptide release from intact isolated ganglia is a reliable method to study the responsiveness of sensory neurons in situ in comparison with neuronal cell cultures.
降钙素基因相关肽（CGRP）和P物质等神经肽在相当比例的初级传入神经元中被发现。这些神经肽以及前列腺素E2的释放是这些初级传入神经激活的一个公认指标。先前的研究使用了酶处理和机械解离的初级传入神经元培养物、新鲜组织切片或组织块。在本研究中，我们证明了完整分离的背根神经节和三叉神经节中CGRP和前列腺素E2的释放。用有害热、低pH值、炎症介质和高钾浓度刺激可增加CGRP的释放。总之，与神经元细胞培养相比，完整分离的神经节中神经肽的释放是一种研究感觉神经元原位反应性的可靠方法。
