Manire Charles A, Stacy Brian A, Kinsel Michael J, Daniel Heather T, Anderson Eric T, Wellehan James F X
Mote Marine Laboratory and Aquarium, 1600 Ken Thompson Parkway, Sarasota, FL 34236, USA.
Vet Microbiol. 2008 Aug 25;130(3-4):227-37. doi: 10.1016/j.vetmic.2008.01.013. Epub 2008 Feb 8.
A subadult loggerhead turtle, Caretta caretta, presented with generalized small, white, raised lesions over its neck, shoulders, and all four flippers. A juvenile green turtle, Chelonia mydas, recently treated for fibropapillomatosis, presented with four similar localized lesions on one flipper. To diagnose the conditions, biopsies of the lesions were taken for histopathology, electron microscopy, and molecular diagnostics. Histopathologic findings were similar in the two turtles and skin lesions were characterized by multifocal areas of epidermal hyperplasia accompanied by variation and abnormalities in the nuclear morphology of keratinocytes and a few intranuclear inclusions in some cells. Transmission electron microscopy revealed multiple epithelial cells with large intranuclear aggregates of virions consistent in morphology with papillomavirus. Papillomavirus was detected in samples from both turtles by polymerase chain reaction (PCR). Sequence analysis of the partial sequence of the papillomavirus E1 gene revealed two viruses (CcPV and CmPV) that were distinct from each other and from other species in Papillomaviridae, and likely represent two novel species and perhaps a new genus.
一只亚成体蠵龟（Caretta caretta）的颈部、肩部和所有四只鳍状肢上出现了广泛的、小的、白色的、凸起的病变。一只幼年绿海龟（Chelonia mydas）最近因纤维乳头瘤病接受治疗，其一只鳍状肢上出现了四个类似的局部病变。为了诊断病情，对病变进行活检以进行组织病理学、电子显微镜检查和分子诊断。两只海龟的组织病理学发现相似，皮肤病变的特征是表皮增生的多灶性区域，伴有角质形成细胞核形态的变化和异常，以及一些细胞中的核内包涵体。透射电子显微镜显示多个上皮细胞含有大量核内病毒粒子聚集体，其形态与乳头瘤病毒一致。通过聚合酶链反应（PCR）在两只海龟的样本中均检测到乳头瘤病毒。乳头瘤病毒E1基因部分序列的序列分析显示，两种病毒（CcPV和CmPV）彼此不同，且与乳头瘤病毒科的其他物种不同，可能代表两个新物种，也许是一个新属。
