Sawczuk Beata, Gołębiewska Maria, Mazurek Andrzej, Chyczewski Lech
Department of Prosthetic Dentistry, Medical University in Bialystok, Poland.
Department if Gynecology, Medical University in Bialystok, Poland.
Prz Menopauzalny. 2017 Mar;16(1):12-18. doi: 10.5114/pm.2017.66178. Epub 2017 Apr 26.
Oestrogens act on target cells through α and β receptors (ERα and ERβ). Expression of oestrogen receptors is associated with the age and menopausal condition of women. The aim of the study was an immunohistochemical evaluation of ERα and ERβ receptors in epithelium of the vaginal mucous membrane of women subjected to different forms of hormonal therapy (HTM). Oestrogen receptors ERα and ERβ were identified using immunohistochemical methods and evaluated in smears of vaginal mucous membranes collected from 60 patients subjected to HTM (including 20 patients after oral therapy, 20 patients after transdermal therapy, and 20 patients after vaginal therapy). The results showed a significant change in immunoreactivity of both studied receptors after three months of hormone therapy. The biggest differences in the changes of intensity of ERα and ERβ reactions were observed in patients subjected to vaginal therapy. Immunostaining for α receptor showed differences between three types of hormone therapy. The highest increase in the overall intensity occurred after three months of topical therapy. Immunostaining for Erβ also varied for different types of hormone therapy. The results indicate that hormone therapy administered vaginally is the most effective in the treatment of urogenital ailments during menopause. In addition, topical therapy eliminates adverse effects of systemic oestrogen.
雌激素通过α和β受体（雌激素受体α和雌激素受体β）作用于靶细胞。雌激素受体的表达与女性的年龄和绝经状态有关。本研究的目的是对接受不同形式激素替代疗法（HTM）的女性阴道黏膜上皮中的雌激素受体α和雌激素受体β进行免疫组织化学评估。采用免疫组织化学方法鉴定雌激素受体α和雌激素受体β，并在60例接受HTM的患者（包括20例口服治疗后患者、20例经皮治疗后患者和20例阴道治疗后患者）的阴道黏膜涂片样本中进行评估。结果显示，激素治疗三个月后，两种研究受体的免疫反应性发生了显著变化。在接受阴道治疗的患者中，观察到雌激素受体α和雌激素受体β反应强度变化的最大差异。α受体的免疫染色显示了三种激素治疗之间的差异。局部治疗三个月后，总体强度增加最高。雌激素受体β的免疫染色在不同类型的激素治疗中也有所不同。结果表明，阴道给药的激素治疗在绝经期间泌尿生殖系统疾病的治疗中最有效。此外，局部治疗消除了全身雌激素的不良反应。
