一种用于小型反刍动物支原体的无血清半限定培养基。
A semi-defined medium without serum for small ruminant mycoplasmas.
作者信息
Ramírez Ana S, Fleitas Juan Luis, Rosales Rubén S, Poveda Carlos, de la Fe Christian, Andrada Marisa, Castro Ayoze, Poveda José B
机构信息
Unidad de Epidemiología y Medicina Preventiva, Spain.
出版信息
Vet J. 2008 Oct;178(1):149-52. doi: 10.1016/j.tvjl.2007.07.023. Epub 2008 Mar 6.
The composition of the medium used to cultivate Mycoplasma species is very important. Serum is one of the most important additives as it contains lipids (cholesterol) and serum proteins, which are essential for the growth of the organisms. This work reports the development of a semi-defined medium, called MWS (Medium Without Serum) produced without animal serum and bovine serum albumin. MWS seems to be suitable for cultivating several species of caprine mycoplasma, especially M. mycoides subsp. mycoides (LC) and M. mycoides subsp. capri.
用于培养支原体属物种的培养基成分非常重要。血清是最重要的添加剂之一，因为它含有脂质（胆固醇）和血清蛋白，这些对生物体的生长至关重要。这项工作报道了一种半限定培养基的开发，称为MWS（无血清培养基），它是在无动物血清和牛血清白蛋白的情况下生产的。MWS似乎适用于培养几种山羊支原体物种，尤其是丝状支原体山羊亚种（LC）和丝状支原体山羊亚种。
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