Danilov N A, Ignatieva N Yu, Iomdina E N, Semenova S A, Rudenskaya G N, Grokhovskaya T E, Lunin V V
Moscow State University, Russia.
Biochim Biophys Acta. 2008 May;1780(5):764-72. doi: 10.1016/j.bbagen.2008.01.014. Epub 2008 Feb 7.
The paper aims at the evaluation of prospects for using glyceraldehyde as a cross-linking agent for the scleral tissue. Stability parameters (denaturation temperature, Young's modulus, ultimate tensile stress, proteolytic resistance) and analytical parameter (fluorescence intensity) were determined during the glycation process of isolated rabbit sclera. The analysis of fluorescence spectral characteristic provided information about some glycation products. The glyceraldehyde treatment was resulted in a significant increase in thermal stability, proteolytic resistance and improvement of biomechanical characteristics (Young's modulus, ultimate tensile stress). Unique properties of the reaction between scleral collagen and glyceraldehyde are observed at short cross-linking times. The appearance of intermediate collagen fraction with lowest thermal and proteolytic stability was detected.
本文旨在评估使用甘油醛作为巩膜组织交联剂的前景。在分离的兔巩膜糖基化过程中测定了稳定性参数（变性温度、杨氏模量、极限拉伸应力、抗蛋白酶解性）和分析参数（荧光强度）。荧光光谱特性分析提供了有关一些糖基化产物的信息。甘油醛处理导致热稳定性、抗蛋白酶解性显著增加，并改善了生物力学特性（杨氏模量、极限拉伸应力）。在短交联时间下观察到巩膜胶原蛋白与甘油醛之间反应的独特性质。检测到具有最低热稳定性和抗蛋白酶解性中间胶原组分的出现。
